2026-10-29T17:40:53Zhttps://riubu.ubu.es/oai/requestoai:riubu.ubu.es:10259/66672024-04-16T12:31:08Zcom_10259_4244com_10259_5086com_10259_2604col_10259_4245
Preparation of Water-in-Oil Nanoemulsions Loaded with Phenolic-Rich Olive Cake Extract Using Response Surface Methodology Approach
Niknam, S. Mehdi
Kashaninejad, Mansoore
Escudero Barbero, Isabel
Sanz Díez, Mª Teresa
Beltrán Calvo, Sagrario
Benito Moreno, José Manuel
Water-in-oil (W/O) nanoemulsion
Response surface methodology
High-energy emulsification
Phenolics
Encapsulation
Stability
In this study, we aimed to prepare stable water-in-oil (W/O) nanoemulsions loaded with a
phenolic-rich aqueous phase from olive cake extract by applying the response surface methodology
and using two methods: rotor-stator mixing and ultrasonic homogenization. The optimal nanoemulsion formulation was 7.4% (w/w) of olive cake extract as the dispersed phase, and 11.2% (w/w) of
a surfactant mixture of polyglycerol polyricinoleate (97%) and Tween 80 (3%) in Miglyol oil as the
continuous phase. Optimum results were obtained by ultrasonication for 15 min at 20% amplitude,
yielding W/O nanoemulsion droplets of 104.9 ± 6.7 nm in diameter and with a polydispersity index
(PDI) of 0.156 ± 0.085. Furthermore, an optimal nanoemulsion with a droplet size of 105.8 ± 10.3 nm
and a PDI of 0.255 ± 0.045 was prepared using a rotor-stator mixer for 10.1 min at 20,000 rpm. High
levels of retention of antioxidant activity (90.2%) and phenolics (83.1–87.2%) were reached after
30 days of storage at room temperature. Both W/O nanoemulsions showed good physical stability
during this storage period.
2022-05-18
2022-05-18
2022-01
info:eu-repo/semantics/article
2304-8158
http://hdl.handle.net/10259/6667
10.3390/foods11030279
2304-8158
eng
Foods. 2022, V. 11, n. 3, 279
https://doi.org/10.3390/foods11030279
info:eu-repo/grantAgreement/Junta de Castilla y León//BU050P20
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-104950RB-I00/ES/VALORIZACION DE SUBPRODUCTOS MARINOS MEDIANTE TECNOLOGIAS DE FLUIDOS SUB- Y SUPERCRITICOS PARA LA OBTENCION DE BIOCOMPUESTOS VALIOSOS
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
Atribución 4.0 Internacional
MDPI