2026-07-19T02:46:42Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/66852022-06-24T11:00:39Zcom_10259_4313com_10259_5086com_10259_2604col_10259_4314

00925njm 22002777a 4500 dc Arnáiz Alonso, Ana author Guembe García, Marta author Delgado-Pinar, Estefanía author Valente, Artur J. M. author Ibeas Cortes, Saturnino author García Pérez, José Miguel author Vallejos Calzada, Saúl author 2022-05 We have faced the preparation of fully water-soluble fluorescent peptide substrate with long-term environmental stability (in solution more than 35 weeks) and, accordingly, with stable results in the use of this probe in determining the activity of enzymes. We have achieved this goal by preparing a co-polymer of the commercial N-vinyl-2-pyrrolidone (99.5% mol) and a fluorescent substrate for trypsin activity determination having a vinylic group (0.5%). The activity of trypsin has been measured in water solutions of this polymer over time, contrasted against the activity of both the commercial substrate Z-L-Arg-7-amido-4-methylcoumarin hydrochloride and its monomeric derivative, prepared ad-hoc. Initially, the activity of the sensory polymer was 74.53 ± 1.72 nmol/min/mg of enzyme, while that of the commercial substrate was 20.44 ± 0.65 nmol/min/mg of enzyme, the former maintained stable along weeks and the latter with a deep decay to zero in three weeks. The ‘protection’ effect exerted by the polymer chain has been studied by solvation studies by UV–Vis spectroscopy, steady-state & time resolved fluorescence, thermogravimetry and isothermal titration calorimetry. http://hdl.handle.net/10259/6685 10.1038/s41598-022-12848-4 2045-2322 Actuators Sensors and biosensors The role of polymeric chains as a protective environment for improving the stability and efficiency of fluorogenic peptide substrates