2026-04-19T12:59:02Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/76162024-07-25T07:49:19Zcom_10259_9476com_10259.4_106com_10259_2604col_10259_9477

Laguna Teno, Félix da8e7aaf-4c3d-47a4-8585-5bbe6587d5b2 600 Suarez Diez, Maria 02e00910-18d5-46be-b42a-e43cca9f9803 600 Tamayo Ramos, Juan Antonio 547 600 0000-0002-7071-002X 2023-03-29T11:29:21Z 2023-03-29T11:29:21Z 2020-08 1664-302X http://hdl.handle.net/10259/7616 10.3389/fmicb.2020.01943 1664-302X Graphene oxide has become a very appealing nanomaterial during the last years for many different applications, but its possible impact in different biological systems remains unclear. Here, an assessment to understand the toxicity of different commercial graphene oxide nanomaterials on the unicellular fungal model organism Saccharomyces cerevisiae was performed. For this task, an RNA purification protocol was optimized to avoid the high nucleic acid absorption capacity of graphene oxide. The developed protocol is based on a sorbitol gradient separation process for the isolation of adequate ribonucleic acid levels (in concentration and purity) from yeast cultures exposed to the carbon derived nanomaterial. To pinpoint potential toxicity mechanisms and pathways, the transcriptome of S. cerevisiae exposed to 160 mg L–1 of monolayer graphene oxide (GO) and graphene oxide nanocolloids (GOC) was studied and compared. Both graphene oxide products induced expression changes in a common group of genes (104), many of them related to iron homeostasis, starvation and stress response, amino acid metabolism and formate catabolism. Also, a high number of genes were only differentially expressed in either GO (236) or GOC (1077) exposures, indicating that different commercial products can induce specific changes in the physiological state of the fungus. This work was supported by the European Union’s H2020 Research and Innovation Programme under the Marie Skłodowska-Curie Grant Agreement Nos. 691095 and 734873 and Junta de Castilla y Leon-FEDER under Grant Nos. BU079U16 and UBU-16-B. application/pdf eng Frontiers Media Frontiers in Microbiology. 2020, V. 11, art. 1943 https://doi.org/10.3389/fmicb.2020.01943 info:eu-repo/grantAgreement/EC/H2020/691095/EU/Developing and implementation of a new generation of nanosafety assessment tools/NANOGENTOOLS/ info:eu-repo/grantAgreement/EC/H2020/734873/EU/CO2 absorbing Materials Project- RISE/CO2MPRISE/ info:eu-repo/grantAgreement/Junta de Castilla y León//BU079U16//Avances en nanobiofísica del ADN, nanofibras y evaluación de la nanoseguridad de materiales/NANOBIO-FIBERSAFE/ info:eu-repo/grantAgreement/Junta de Castilla y León//UBU-16-B/ Atribución 4.0 Internacional http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess Saccharomyces cerevisiae Biological response Commercial graphene oxide Chelating agent RNA isolation Transcriptomics Differential expression Microbiología Materiales Microbiology Materials Commonalities and Differences in the Transcriptional Response of the Model Fungus Saccharomyces cerevisiae to Different Commercial Graphene Oxide Materials info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Frontiers in Microbiology 11 THUMBNAIL Laguna-fm_2020.pdf.jpg Laguna-fm_2020.pdf.jpg IM Thumbnail image/jpeg 3260 https://riubu.ubu.es/bitstream/10259/7616/4/Laguna-fm_2020.pdf.jpg 35f8a62861c95f837e64dddf23985eb9 MD5 4 LICENSE license.txt license.txt text/plain; charset=utf-8 998 https://riubu.ubu.es/bitstream/10259/7616/3/license.txt aa90105e1c90a1b4fe810649aa967b7d MD5 3 CC-LICENSE license_rdf license_rdf application/rdf+xml; charset=utf-8 908 https://riubu.ubu.es/bitstream/10259/7616/2/license_rdf 0175ea4a2d4caec4bbcc37e300941108 MD5 2 ORIGINAL Laguna-fm_2020.pdf Laguna-fm_2020.pdf application/pdf 2838334 https://riubu.ubu.es/bitstream/10259/7616/1/Laguna-fm_2020.pdf 40ae8f961be8eadae2bc04d49553f881 MD5 1 10259/7616 oai:riubu.ubu.es:10259/7616 2024-07-25 09:49:19.543 Repositorio Institucional de la Universidad de Burgos bubrep@ubu.es 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