2026-05-28T17:24:42Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/80542023-11-20T08:57:24Zcom_10259_5827com_10259_5086com_10259_2604col_10259_5828

00925njm 22002777a 4500 dc Gerardi, Gisela author Cavia Saiz, Mónica author Rivero Pérez, Maria Dolores author González San José, Mª Luisa author Muñiz Rodríguez, Pilar author 2019-07 Wine by-products show great potential as source of bioactive compounds that protect the vascular endothelial function by the modulation of both Nrf2 and Nf-κB pathways. This study investigates the pathways involved in the effects of a red wine pomace product (rWPP) against inflammatory and oxidative damage in hyperglycemic EA.hy926 endothelial cells. rWPP-digested fractions showed an inhibitory effect on IKK/IκBα/NF-κB pathway and a stimulatory effect on Akt-p38-MAPK/Nrf2 pathway with an impact on their antioxidant and anti-inflammatory downstream targets. In addition, the pathways regulation was also accompanied by downregulation of the gene expression of superoxide dismutase 2, cyclooxygenase 2 and NADPH oxidase 4. These results suggest the expression of SOD2 as an early adaptive response to the inflammatory effect mediated by NF-κB in hyperglycemic cells, and the treatment with the rWPP-digested fractions regulate this inflammatory process by Nrf2 pathway increased expression of antioxidant enzymes. 1756-4646 1756-4646 http://hdl.handle.net/10259/8054 10.1016/j.jff.2019.05.003 Polyphenols Wine pomace Endothelial dysfunction NF-κB Nrf2 p38MAPK Akt SIRT1 Oxidative stress EA.hy926 Phase 2 enzymes SOD2 NOX4 COX2 Modulation of Akt-p38-MAPK/Nrf2/SIRT1 and NF-κB pathways by wine pomace product in hyperglycemic endothelial cell line