2026-06-29T07:01:01Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/80692023-11-22T01:05:28Zcom_10259_5827com_10259_5086com_10259_2604col_10259_5828

00925njm 22002777a 4500 dc Durán Fernández-Feijóo, Cristina author Rodríguez-Fanjul, Javier author López-Abat, Miriam author Hadley, Stephanie author Cavia Saiz, Mónica author Muñiz Rodríguez, Pilar author Arnaez, Juan author Fernández-Lorenzo, José Ramón author Camprubí Camprubí, Marta author 2021-09 Hypoxic ischemic encephalopathy (HIE) is one of the main causes of morbidity and mortality during the neonatal period, despite treatment with hypothermia. There is evidence that oxidative damage plays an important role in the pathophysiology of hypoxic-ischemic (HI) brain injury. Our aim was to investigate whether postnatal allopurinol administration in combination with hypothermia would reduce oxidative stress (OS) biomarkers in an animal model of HIE. Postnatal 10-day rat pups underwent unilateral HI of moderate severity. Pups were randomized into: Sham operated, hypoxic-ischemic (HI), HI + allopurinol (HIA), HI + hypothermia (HIH), and HI + hypothermia + allopurinol (HIHA). Biomarkers of OS and antioxidants were evaluated: GSH/GSSG ratio and carbonyl groups were tested in plasma. Total antioxidant capacity (TAC) was analyzed in plasma and cerebrospinal fluid, and 8-iso-prostaglandin F2α was measured in brain tissue. Plasma 2,20–azinobis- (3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS) levels were preserved in those groups that received allopurinol and dual therapy. In cerebrospinal fluid, only the HIA group presented normal ferric reducing ability of plasma (FRAP) levels. Protein oxidation and lipid peroxidation were significantly reduced in all groups treated with hypothermia and allopurinol, thus enhancing neuroprotection in HIE. http://hdl.handle.net/10259/8069 10.3390/antiox10101523 2076-3921 Allopurinol Hypothermia Hypoxic-ischemic encephalopathy Oxidative stress Oxidative damage Effects of Hypothermia and Allopurinol on Oxidative Status in a Rat Model of Hypoxic Ischemic Encephalopathy