2026-06-29T12:06:12Zhttps://riubu.ubu.es/oai/requestoai:riubu.ubu.es:10259/80692023-11-22T01:05:28Zcom_10259_5827com_10259_5086com_10259_2604col_10259_5828
Repositorio Institucional de la Universidad de Burgos
author
Durán Fernández-Feijóo, Cristina
author
Rodríguez-Fanjul, Javier
author
López-Abat, Miriam
author
Hadley, Stephanie
author
Cavia Saiz, Mónica
author
Muñiz Rodríguez, Pilar
author
Arnaez, Juan
author
Fernández-Lorenzo, José Ramón
author
Camprubí Camprubí, Marta
2023-11-21T08:12:55Z
2023-11-21T08:12:55Z
2021-09
http://hdl.handle.net/10259/8069
10.3390/antiox10101523
2076-3921
Hypoxic ischemic encephalopathy (HIE) is one of the main causes of morbidity and mortality during the neonatal period, despite treatment with hypothermia. There is evidence that oxidative
damage plays an important role in the pathophysiology of hypoxic-ischemic (HI) brain injury. Our
aim was to investigate whether postnatal allopurinol administration in combination with hypothermia would reduce oxidative stress (OS) biomarkers in an animal model of HIE. Postnatal 10-day rat
pups underwent unilateral HI of moderate severity. Pups were randomized into: Sham operated,
hypoxic-ischemic (HI), HI + allopurinol (HIA), HI + hypothermia (HIH), and HI + hypothermia
+ allopurinol (HIHA). Biomarkers of OS and antioxidants were evaluated: GSH/GSSG ratio and
carbonyl groups were tested in plasma. Total antioxidant capacity (TAC) was analyzed in plasma and
cerebrospinal fluid, and 8-iso-prostaglandin F2α was measured in brain tissue. Plasma 2,20–azinobis-
(3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS) levels were preserved in those groups that received
allopurinol and dual therapy. In cerebrospinal fluid, only the HIA group presented normal ferric
reducing ability of plasma (FRAP) levels. Protein oxidation and lipid peroxidation were significantly
reduced in all groups treated with hypothermia and allopurinol, thus enhancing neuroprotection
in HIE.
eng
Atribución 4.0 Internacional
Allopurinol
Hypothermia
Hypoxic-ischemic encephalopathy
Oxidative stress
Oxidative damage
Effects of Hypothermia and Allopurinol on Oxidative Status in a Rat Model of Hypoxic Ischemic Encephalopathy
info:eu-repo/semantics/article
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URL
https://riubu.ubu.es/bitstream/10259/8069/1/Dur%c3%a1n-antioxidants_2021.pdf
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Durán-antioxidants_2021.pdf