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oai:riubu.ubu.es:10259/81922023-12-13T01:05:23Zcom_10259.4_2516com_10259_5086com_10259_2604col_10259.4_2517

Pilar Izquierdo, María Concepción López Fouz, María Ortega Santamaría, Natividad Busto Núñez, Mª Dolores 2023-12-12T11:19:12Z 2023-12-12T11:19:12Z 2023-08 0175-7598 http://hdl.handle.net/10259/8192 10.1007/s00253-023-12724-9 1432-0614 Debittering of citrus by-products is required to obtain value-added compounds for application in the food industry (e.g., dietary fber, bioactive compounds). In this work, the immobilization of Rhodococcus fascians cells by encapsulation in Ca-alginate hollow beads and entrapment in poly(vinyl alcohol)/polyethylene glycol (PVA/PEG) cryogels was studied as an alternative to chemical treatments for degrading the bitter compound limonin. Previously, the Rhodococcus strain was adapted using orange peel extract to increase its tolerance to limonoids. The optimal conditions for the encapsulation of microbial cells were 2% Na-alginate, 4% CaCl2, 4% carboxymethylcellulose (CMC), and a microbial load of 0.6 OD600 (optical density at 600 nm). For immobilization by entrapment, the optimal conditions were 8% PVA, 8% PEG, and 0.6 OD600 microbial load. Immobilization by entrapment protected microbial cells better than encapsulation against the citrus medium stress conditions (acid pH and composition). Thus, under optimal immobilization conditions, limonin degradation was 32 and 28% for immobilization in PVA/PEG gels and in hollow beads, respectively, in synthetic juice (pH 3) after 72 h at 25 °C. Finally, the microbial cells entrapped in the cryogels showed a higher operational stability in orange juice than the encapsulated cells, with four consecutive cycles of reuse (runs of 24 h at 25 °C). application/pdf eng Springer Nature Applied Microbiology and Biotechnology. 2023, V. 107, n. 20, p. 6377-6388 https://doi.org/10.1007/s00253-023-12724-9 Atribución 4.0 Internacional http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess Cell immobilization Ca-alginate hollow beads PVA/PEG cryogels Rhodococcus Debittering Citrus by-products Bioquímica Alimentos Biochemistry Food Immobilization of Rhodococcus by encapsulation and entrapment: a green solution to bitter citrus by-products info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Applied Microbiology and Biotechnology 107 20 6377 6388