2026-06-10T19:47:32Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/81932023-12-13T01:05:24Zcom_10259.4_2516com_10259_5086com_10259_2604col_10259.4_2517

Barley seed coating with urease and phosphatase to improve N and P uptake Mvila, Beaufray Gilaime Pilar Izquierdo, María Concepción Busto Núñez, Mª Dolores Pérez Mateos, Manuel Ortega Santamaría, Natividad Soil enzymes Seed encapsulation Plant nutrients uptake Humic acids Bioquímica Alimentos Biochemistry Food In this work, barley seed coating with urease and phosphatase, free and immobilized in humic acids, was studied as a tool to enhance the use efficiency of N and P by plants. The seeds were encapsulated in gels of Ca-alginate by a method that allows gelation of the polymer, containing enzymes, from the surface of seeds outward. The coating process did not affect seed germination. A pot experiment with the coated seeds was carried out. The seed coating with free enzymes increased soil ammonium-N (NH4+-N) and inorganic P up to 39 and 54 %, respectively, and up to 62 and 64 %, with the immobilized enzymes, in comparison with control pots. Seed coating improved N (47-137 %) and P (29-61 %) uptake by plants. Moreover, a synergistic response in N and P uptake with both urease and phosphatase was observed. It can be concluded that the seed coating with urease and phosphatase exerted a positive effect enhancing the use of N and P by plants, which could lead to the development of natural fertilizers, minimizing the application of chemical fertilizers. 2023-12-12T11:19:30Z 2023-12-12T11:19:30Z 2020-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/10259/8193 10.1590/1678-992X-2018-0227 1678-992X eng Scientia Agricola. 2020, V. 77, n. 6 https://doi.org/10.1590/1678-992X-2018-0227 Atribución 4.0 Internacional http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess application/pdf Universidade de São Paulo. Escola Superior de Agricultura Luiz de Queiroz