2026-06-02T01:51:12Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/83082024-01-12T10:43:50Zcom_10259.4_2516com_10259_5086com_10259_2604col_10259.4_2517

Ordóñez Robles, María Santos Beneit, Fernando Albillos García, Silvia María Liras, Paloma Martín, Juan F. Rodríguez García, Antonio 2024-01-11T19:35:21Z 2024-01-11T19:35:21Z 2017 0175-7598 http://hdl.handle.net/10259/8308 10.1007/s00253-017-8545-5 1432-0614 In this work, we identified glucose and glycerol as tacrolimus repressing carbon sources in the important species Streptomyces tsukubaensis. A genome-wide analysis of the transcriptomic response to glucose and glycerol additions was performed using microarray technology. The transcriptional time series obtained allowed us to compare the transcriptomic profiling of S. tsukubaensis growing under tacrolimus producing and non-producing conditions. The analysis revealed important and different metabolic changes after the additions and a lack of transcriptional activation of the fkb cluster. In addition, we detected important differences in the transcriptional response to glucose between S. tsukubaensis and the model species Streptomyces coelicolor. A number of genes encoding key players of morphological and biochemical differentiation were strongly and permanently downregulated by the carbon sources. Finally, we identified several genes showing transcriptional profiles highly correlated to that of the tacrolimus biosynthetic pathway regulator FkbN that might be potential candidates for the improvement of tacrolimus production. This work was supported by the European Union through an ERA-IB (PIM2010EEI-00677) international cooperation project. M. Ordóñez-Robles received a FPU fellowship of the Ministerio de Educación y Ciencia (Spain). application/pdf eng Springer Applied Microbiology and Biotechnology. 2017, V. 101, n. 22, p. 8181-8195 https://doi.org/10.1007/s00253-017-8545-5 Streptomycestsukubaensis Tacrolimus FK506 Carbon regulation Transcriptomics Microbiología Biotecnología Biología Microbiology Biotechnology Biology Streptomyces tsukubaensis as a new model for carbon repression: transcriptomic response to tacrolimus repressing carbon sources info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion info:eu-repo/semantics/openAccess Applied Microbiology and Biotechnology 101 22 8181 8195