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oai:riubu.ubu.es:10259/83282024-01-15T10:41:26Zcom_10259_3592com_10259_3591com_10259.4_106com_10259_2604com_10259_3924com_10259_5086col_10259_3930col_10259_3925

Fiore, Michele García Valverde, María Carreira Barral, Israel Moran, Óscar 2024-01-13T19:35:33Z 2024-01-13T19:35:33Z 2020 0014-2999 http://hdl.handle.net/10259/8328 10.1016/j.ejphar.2020.173592 1879-0712 Prodiginines and tambjamines are anion-selective ionophores capable of facilitating the transport of anions across the plasma membrane in mammalian cells. One of the potential applications of these anionophores is the possibility of employing them as a substitutive therapy for pathologies involving anion channels, as in cystic fibrosis. We have studied the interaction of a large anion as gluconate with three prodiginine- and two tambjamine-like compounds. Apparent dissociation constants for the chloride, iodide and gluconate complexes were estimated from iodide influx experiments in mammalian cells exposed to different extracellular anion combinations. Our experiments indicate that gluconate is not transported by the prodiginines, leaving the anionophores free to transport chloride and iodide. Conversely, gluconate would be transported to some extent by the tambjamines, competing with halides for the anionophores, and consequently reducing their flux. This might be related to the different structural features of both families of compounds. These data have important implications for the selection of impermeable anions in the analysis of the anionophore mechanism. This work has received funding from the European Union's Horizon 2020 research and innovation program under grant agreement No 667079. application/pdf eng Elsevier European Journal of Pharmacology. 2020, V. 889, 173592 https://doi.org/10.1016/j.ejphar.2020.173592 Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess Anionophore Anion transport Anion binding Prodiginine Tambjamine Química orgánica Chemistry, Organic The different anion transport capability of prodiginine- and tambjamine-like molecules info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion European Journal of Pharmacology 889 173592