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oai:riubu.ubu.es:10259/83282024-01-15T10:41:26Zcom_10259_3592com_10259_3591com_10259.4_106com_10259_2604com_10259_3924com_10259_5086col_10259_3930col_10259_3925

00925njm 22002777a 4500 dc Fiore, Michele author García Valverde, María author Carreira Barral, Israel author Moran, Óscar author 2020 Prodiginines and tambjamines are anion-selective ionophores capable of facilitating the transport of anions across the plasma membrane in mammalian cells. One of the potential applications of these anionophores is the possibility of employing them as a substitutive therapy for pathologies involving anion channels, as in cystic fibrosis. We have studied the interaction of a large anion as gluconate with three prodiginine- and two tambjamine-like compounds. Apparent dissociation constants for the chloride, iodide and gluconate complexes were estimated from iodide influx experiments in mammalian cells exposed to different extracellular anion combinations. Our experiments indicate that gluconate is not transported by the prodiginines, leaving the anionophores free to transport chloride and iodide. Conversely, gluconate would be transported to some extent by the tambjamines, competing with halides for the anionophores, and consequently reducing their flux. This might be related to the different structural features of both families of compounds. These data have important implications for the selection of impermeable anions in the analysis of the anionophore mechanism. 0014-2999 http://hdl.handle.net/10259/8328 10.1016/j.ejphar.2020.173592 1879-0712 Anionophore Anion transport Anion binding Prodiginine Tambjamine The different anion transport capability of prodiginine- and tambjamine-like molecules