2026-06-21T17:38:58Zhttps://riubu.ubu.es/oai/requestoai:riubu.ubu.es:10259/83282024-01-15T10:41:26Zcom_10259_3592com_10259_3591com_10259.4_106com_10259_2604com_10259_3924com_10259_5086col_10259_3930col_10259_3925
Repositorio Institucional de la Universidad de Burgos
author
Fiore, Michele
author
García Valverde, María
author
Carreira Barral, Israel
author
Moran, Óscar
2024-01-13T19:35:33Z
2024-01-13T19:35:33Z
2020
0014-2999
http://hdl.handle.net/10259/8328
10.1016/j.ejphar.2020.173592
1879-0712
Prodiginines and tambjamines are anion-selective ionophores capable of facilitating the transport of anions across the plasma membrane in mammalian cells. One of the potential applications of these anionophores is the possibility of employing them as a substitutive therapy for pathologies involving anion channels, as in cystic fibrosis. We have studied the interaction of a large anion as gluconate with three prodiginine- and two tambjamine-like compounds. Apparent dissociation constants for the chloride, iodide and gluconate complexes were estimated from iodide influx experiments in mammalian cells exposed to different extracellular anion combinations. Our experiments indicate that gluconate is not transported by the prodiginines, leaving the anionophores free to transport chloride and iodide. Conversely, gluconate would be transported to some extent by the tambjamines, competing with halides for the anionophores, and consequently reducing their flux. This might be related to the different structural features of both families of compounds. These data have important implications for the selection of impermeable anions in the analysis of the anionophore mechanism.
eng
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Anionophore
Anion transport
Anion binding
Prodiginine
Tambjamine
The different anion transport capability of prodiginine- and tambjamine-like molecules
info:eu-repo/semantics/article
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URL
https://riubu.ubu.es/bitstream/10259/8328/1/Fiore-ejp_2020.pdf
File
MD5
b206ff7dc051cc1e693901688b68c883
751047
application/pdf
Fiore-ejp_2020.pdf