2026-06-18T03:24:59Zhttps://riubu.ubu.es/oai/requestoai:riubu.ubu.es:10259/83772024-01-18T01:05:23Zcom_10259_4862com_10259_5086com_10259_2604col_10259_4863
Repositorio Institucional de la Universidad de Burgos
author
Hai, Rong
author
Martínez Sobrido, Luis
author
Fraser, Kathryn A.
author
Ayllón Barasoain, Juan
author
García Sastre, Adolfo
author
Palese, Peter
2024-01-17T13:11:12Z
2024-01-17T13:11:12Z
2008-11
0022-538X
http://hdl.handle.net/10259/8377
10.1128/jvi.01213-08
1098-5514
Type B influenza viruses can cause substantial morbidity and mortality in the population, and vaccinationremains by far the best means of protection against infections with these viruses. Here, we report theconstruction of mutant influenza B viruses for potential use as improved live-virus vaccine candidates.Employing reverse genetics, we altered the NS1 gene, which encodes a type I interferon (IFN) antagonist. Theresulting NS1 mutant viruses induced IFN and, as a consequence, were found to be attenuated in vitro and invivo. The absence of pathogenicity of the NS1 mutants in both BALB/c and C57BL/6 PKR / mice wasconfirmed. We also provide evidence that influenza B virus NS1 mutants induce a self-adjuvanted immuneresponse and confer effective protection against challenge with both homologous and heterologous B virusstrains in mice.
eng
Influenza B Virus NS1-Truncated Mutants: Live-Attenuated Vaccine Approach
info:eu-repo/semantics/article
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
URL
https://riubu.ubu.es/bitstream/10259/8377/1/Hai-jv_2008.pdf
File
MD5
37cbf759deaa12c61cafa34005c75e54
1985544
application/pdf
Hai-jv_2008.pdf