2026-07-09T19:18:17Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/85512024-02-02T01:05:25Zcom_10259.4_2516com_10259_5086com_10259_2604com_10259_4244col_10259.4_2517col_10259_4245

González Temiño, Yaiza Ruiz Pérez, María Olga Ortega Santamaría, Natividad Ramos Gómez, Sonia Busto Núñez, Mª Dolores 2024-02-01T13:08:25Z 2024-02-01T13:08:25Z 2021-10 1466-8564 http://hdl.handle.net/10259/8551 10.1016/j.ifset.2021.102790 An enzymatic membrane reactor (EMR) is assembled for the immobilization of naringinase on a polyethersulfone ultrafiltration membrane, based on fouling-induced method. The effects of molecular weight cut-off, membrane configuration, applied pressure, enzyme concentration and pH are studied in terms of permeate rate, immobilization efficiency, and biocatalytic conversion. The 10 kDa membrane operating in reverse mode, 0.2 MPa, 0.3 gL−1 of enzyme in acetate buffer at pH 5 and cross-linking with 0.25% glutaraldehyde showed the highest naringin conversion (73%). It was determined that the intermediate pore blocking model was the predominant fouling mechanism for the enzymatic immobilization. The EMR was applied for debittering of grapefruit juice, achieving a conversion of naringin below bitterness threshold and maintaining the antioxidant capacity of the juice. Furthermore, the biocatalytic activity of immobilized enzyme was retained at a high level at least during three consecutive reaction runs, with overnight storage at 4 °C after each run. application/pdf eng Elsevier Innovative Food Science & Emerging Technologies. 2021, V. 73, 102790 https://doi.org/10.1016/j.ifset.2021.102790 Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess Enzymatic membrane reactor Naringinase Polyethersulfone membrane Crosslinking Debittering Grapefruit juice Bioquímica Alimentos Ingeniería química Biochemistry Food Chemical engineering Immobilization of naringinase on asymmetric organic membranes: Application for debittering of grapefruit juice info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Innovative Food Science & Emerging Technologies 73 102790