2026-07-09T18:45:30Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/85512024-02-02T01:05:25Zcom_10259.4_2516com_10259_5086com_10259_2604com_10259_4244col_10259.4_2517col_10259_4245

González Temiño, Yaiza 602 600 0000-0002-5456-3760 Ruiz Pérez, María Olga 503 600 0000-0003-0399-092X Ortega Santamaría, Natividad 405 600 0000-0001-6269-7577 Ramos Gómez, Sonia 850 600 0000-0001-8056-3900 Busto Núñez, Mª Dolores 82 600 0000-0003-1647-7850 2024-02-01T13:08:25Z 2024-02-01T13:08:25Z 2021-10 1466-8564 http://hdl.handle.net/10259/8551 10.1016/j.ifset.2021.102790 An enzymatic membrane reactor (EMR) is assembled for the immobilization of naringinase on a polyethersulfone ultrafiltration membrane, based on fouling-induced method. The effects of molecular weight cut-off, membrane configuration, applied pressure, enzyme concentration and pH are studied in terms of permeate rate, immobilization efficiency, and biocatalytic conversion. The 10 kDa membrane operating in reverse mode, 0.2 MPa, 0.3 gL−1 of enzyme in acetate buffer at pH 5 and cross-linking with 0.25% glutaraldehyde showed the highest naringin conversion (73%). It was determined that the intermediate pore blocking model was the predominant fouling mechanism for the enzymatic immobilization. The EMR was applied for debittering of grapefruit juice, achieving a conversion of naringin below bitterness threshold and maintaining the antioxidant capacity of the juice. Furthermore, the biocatalytic activity of immobilized enzyme was retained at a high level at least during three consecutive reaction runs, with overnight storage at 4 °C after each run. application/pdf eng Elsevier Innovative Food Science & Emerging Technologies. 2021, V. 73, 102790 https://doi.org/10.1016/j.ifset.2021.102790 Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess Enzymatic membrane reactor Naringinase Polyethersulfone membrane Crosslinking Debittering Grapefruit juice Bioquímica Alimentos Ingeniería química Biochemistry Food Chemical engineering Immobilization of naringinase on asymmetric organic membranes: Application for debittering of grapefruit juice info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Innovative Food Science & Emerging Technologies 73 102790 THUMBNAIL Gonzalez-ifset_2021.pdf.jpg Gonzalez-ifset_2021.pdf.jpg IM Thumbnail image/jpeg 4995 https://riubu.ubu.es/bitstream/10259/8551/4/Gonzalez-ifset_2021.pdf.jpg 47d8f752a3f7212d2c787e7c5213eb55 MD5 4 LICENSE license.txt license.txt text/plain; charset=utf-8 998 https://riubu.ubu.es/bitstream/10259/8551/3/license.txt aa90105e1c90a1b4fe810649aa967b7d MD5 3 CC-LICENSE license_rdf license_rdf application/rdf+xml; charset=utf-8 805 https://riubu.ubu.es/bitstream/10259/8551/2/license_rdf 4460e5956bc1d1639be9ae6146a50347 MD5 2 ORIGINAL Gonzalez-ifset_2021.pdf Gonzalez-ifset_2021.pdf application/pdf 740787 https://riubu.ubu.es/bitstream/10259/8551/1/Gonzalez-ifset_2021.pdf 0ddb9aca803807e8d4a54e804f817ebb MD5 1 10259/8551 oai:riubu.ubu.es:10259/8551 2024-02-02 02:05:25.489 Repositorio Institucional de la Universidad de Burgos bubrep@ubu.es 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