2026-04-21T03:34:34Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/92842024-06-19T00:05:15Zcom_10259_4292com_10259_5086com_10259_2604col_10259_4293

Ortega Sanz, Irene 4fcf6553-8474-464b-8733-0e84571ee49b 600 Bocigas, Carolina c501a59c-0f3b-4950-83ad-82c6acdf72ec 600 Melero Gil, Beatriz 356 600 0000-0001-8982-8186 Rovira Carballido, Jordi 490 600 0000-0001-6664-9484 2024-06-18T11:12:51Z 2024-06-18T11:12:51Z 2024-02 0740-0020 http://hdl.handle.net/10259/9284 10.1016/j.fm.2023.104397 The high incidence and prevalence of Campylobacter spp. in the food supply chain entail the importance to understand their mechanisms developed to withstand harsh environmental conditions encountered. Different stress conditions and phenotypic approaches were evaluated to study the behaviour of five clinical C. jejuni isolates with different genotypes, including the tolerance to oxygen and the oxidants hydrogen peroxide and cumene hydroperoxide, the motility and the ability to form biofilm on polystyrene and stainless steel at different temperatures and atmospheres. Whole Genome Sequencing was performed to analyse the occurrence of 216 genes involved in these mechanisms plus phase variation. The isolates showed high tolerance to oxygen and peroxide stress with different swimming motility performances and biofilm formation abilities. Aerotolerance was related with a reduced sensitive to peroxide stress and a loss of motility that promotes biofilm formation depending on the material surface. Comparative genomics did not reveal any clear gene pattern, although phase variation occurring during host infection was observed to be crucial for the modulation of the different survival mechanisms adopted by the bacteria. These findings reveal that the bacteria can combine diverse and complex strategies in an efficient manner to survive and persist in the environment. This work was supported by “La Caixa” Foundation and Caja Burgos Foundation, under agreement LCF/PR/PR18/51130007. Ortega-Sanz I received a predoctoral grant from the Junta of Castile and León, cofinanced by the Ministry of Education of the Government of Castile and León and the European Social Fund. application/pdf eng Elsevier Food Microbiology. 2024, V. 117, 104397 https://doi.org/10.1016/j.fm.2023.104397 Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess Campylobacteriosis Aerotolerance Oxidative stress Motility Biofilm formation Genomics Biotecnología Alimentos Biotechnology Food Phase variation modulates the multi-phenotypes displayed by clinical Campylobacter jejuni strains info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Food Microbiology 117 THUMBNAIL Ortega-fm_2024.pdf.jpg Ortega-fm_2024.pdf.jpg IM Thumbnail image/jpeg 5026 https://riubu.ubu.es/bitstream/10259/9284/4/Ortega-fm_2024.pdf.jpg 90c2019005262dc6b82c67f3e4b2a000 MD5 4 LICENSE license.txt license.txt text/plain; charset=utf-8 999 https://riubu.ubu.es/bitstream/10259/9284/3/license.txt b295bcbce42e2caabeb0c623d3860c06 MD5 3 CC-LICENSE license_rdf license_rdf application/rdf+xml; charset=utf-8 805 https://riubu.ubu.es/bitstream/10259/9284/2/license_rdf 4460e5956bc1d1639be9ae6146a50347 MD5 2 ORIGINAL Ortega-fm_2024.pdf Ortega-fm_2024.pdf application/pdf 2795607 https://riubu.ubu.es/bitstream/10259/9284/1/Ortega-fm_2024.pdf 2eca8d465c96e4828d326ccd446e53fa MD5 1 10259/9284 oai:riubu.ubu.es:10259/9284 2024-06-19 02:05:15.919 Repositorio Institucional de la Universidad de Burgos bubrep@ubu.es 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