2026-05-04T08:44:58Zhttps://riubu.ubu.es/oai/requestoai:riubu.ubu.es:10259/95262024-09-05T00:05:21Zcom_10259_4862com_10259_5086com_10259_2604col_10259_4863
Repositorio Institucional de la Universidad de Burgos
author
Cuadrado-Castano, Sara
author
Ayllón Barasoain, Juan
author
Mansour, Mena
author
Iglesia-Vicente, Janis de la
author
Jordan, Stefan
author
Tripathi, Shashank
author
García Sastre, Adolfo
author
Villar, Enrique
2024-09-04T10:40:27Z
2024-09-04T10:40:27Z
2015-05
1535-7163
http://hdl.handle.net/10259/9526
10.1158/1535-7163.MCT-14-0913
1538-8514
Newcastle disease virus (NDV) is considered a promising agent for cancer therapy due to its oncolytic properties. These include preferential replication in transformed cells, induction of innate and adaptive immune responses within tumors, and cytopathic effects in infected tumor cells due to the activation of apoptosis. To enhance the latter and thus possibly enhance the overall oncolytic activity of NDV, we generated a recombinant NDV encoding the human TNF receptor Fas (rNDV-B1/Fas). rNDV-B1/Fas replicates to similar titers as its wild-type (rNDV-B1) counterpart; however, overexpression of Fas in infected cells leads to higher levels of cytotoxicity correlated with faster and increased apoptosis responses, in which both the intrinsic and extrinsic pathways are activated earlier. Furthermore, in vivo studies in syngeneic murine melanoma models show an enhancement of the oncolytic properties of rNDV-B1/Fas, with major improvements in survival and tumor remission. Altogether, our data suggest that upregulation of the proapoptotic function of NDV is a viable approach to enhance its antitumor properties and adds to the currently known, rationally based strategies to design optimized therapeutic viral vectors for the treatment of cancer.
eng
Fas
Apoptosis
Oncolytic
Virotherapy
Recombinant virus
Enhancement of the Proapoptotic Properties of Newcastle Disease Virus Promotes Tumor Remission in Syngeneic Murine Cancer Models
info:eu-repo/semantics/article
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URL
https://riubu.ubu.es/bitstream/10259/9526/1/Cuadrado-mct_2015.pdf
File
MD5
50f2b5962a5b2558b8449ec9451763cc
2715482
application/pdf
Cuadrado-mct_2015.pdf