2026-06-21T19:41:22Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/95282024-09-05T00:05:15Zcom_10259_4862com_10259_5086com_10259_2604col_10259_4863

Ayllón Barasoain, Juan Russell, Rupert J. García Sastre, Adolfo Hale, Benjamin G. 2024-09-04T11:46:37Z 2024-09-04T11:46:37Z 2012-12 0022-538X http://hdl.handle.net/10259/9528 10.1128/jvi.02237-12 Conserved tryptophan-187 facilitates homodimerization of the influenza A virus NS1 protein effector domain. We generated a mutant influenza virus strain expressing NS1-W187R to destabilize this self-interaction. NS1-W187R protein exhibited lower double-stranded RNA (dsRNA)-binding activity, showed a temporal redistribution during infection, and was minimally compromised for interferon antagonism. The mutant virus replicated similarly to the wild type in vitro, but it was slightly attenuated for replication in mice, causing notably reduced morbidity and mortality. These data suggest biological relevance for the W187-mediated homotypic interaction of NS1. This work was supported by NIH funding to A.G.-S. (under grantsR01AI046954 and U19AI083025, and CRIP, Center for Research on In-fluenza Pathogenesis, an NIAID Center of Excellence for Influenza Re-search and Surveillance [CEIRS], contract HHSN266200700010C). Con-focal laser scanning microscopy was performed at the MSSM-MicroscopyShared Resource Facility, supported with funding from a National Insti-tutes of Health-National Cancer Institute (NIH-NCI) shared resourcesgrant (5R24 CA095823-04), an NSF Major Research Instrumentationgrant (DBI-9724504), and an NIH shared instrumentation grant (1 S10RR0 9145-01) application/pdf eng American Society for Microbiology Journal of Virology. 2012, V. 86, n. 23, p. 13095–13098 https://doi.org/10.1128/jvi.02237-12 info:eu-repo/grantAgreement/NIH//R01AI04695/US/ info:eu-repo/grantAgreement/NIH//U19AI08302/US/ info:eu-repo/grantAgreement/NIAID//HHSN266200700010C/US/ info:eu-repo/grantAgreement/NCI//5R24 CA095823-04/US/ info:eu-repo/grantAgreement/NSF//DBI-9724504/US/ info:eu-repo/grantAgreement/NIH//1 S10 RR0 9145-01/US/ Medicina Salud Microbiología Enfermedades infecciosas Medicine Health Microbiology Communicable diseases Contribution of NS1 Effector Domain Dimerization to Influenza A Virus Replication and Virulence info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/openAccess