2026-05-04T23:14:56Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/95292024-09-05T00:05:21Zcom_10259_4862com_10259_5086com_10259_2604col_10259_4863

Laurent-Rolle, Maudry d326b542-d386-4196-8591-c0184ef7e4ae 500 Morrison, Juliet 5424afd2-054c-4beb-ae94-4beb3a3e472d 500 Rajsbaum, Ricardo 0cf7967e-233d-4bf0-b44d-acd237723f48 600 Macleod, Jesica M. Levingston a0c08e6d-a6f1-477c-8f15-d03d25213c9a Pisanelli, Giuseppe 209796b0-7704-41a9-a05c-72f90282ad31 Pham, Alissa 98118799-d4f2-430b-af7f-39f58647c78c Ayllón Barasoain, Juan 852 600 0000-0002-7556-8106 Miorin, Lisa 059f762f-4d32-4718-a378-689c1914c061 600 Martínez Romero, Carles 4f8a8290-2a6e-440b-97fa-12f7e7981a5b 600 tenOever, Benjamin R. 4fe3416c-8a70-402b-95ef-fab15b758379 600 García Sastre, Adolfo 9de2ea8b-c019-414d-bb90-b1d51ef5bbd7 600 2024-09-04T12:21:05Z 2024-09-04T12:21:05Z 2014-09 1931-3128 http://hdl.handle.net/10259/9529 10.1016/j.chom.2014.07.015 To successfully establish infection, flaviviruses have to overcome the antiviral state induced by type I interferon (IFN-I). The nonstructural NS5 proteins of several flaviviruses antagonize IFN-I signaling. Here we show that yellow fever virus (YFV) inhibits IFN-I signaling through a unique mechanism that involves binding of YFV NS5 to the IFN-activated transcription factor STAT2 only in cells that have been stimulated with IFN-I. This NS5-STAT2 interaction requires IFN-I-induced tyrosine phosphorylation of STAT1 and the K63-linked polyubiquitination at a lysine in the N-terminal region of YFV NS5. We identified TRIM23 as the E3 ligase that interacts with and polyubiquitinates YFV NS5 to promote its binding to STAT2 and trigger IFN-I signaling inhibition. Our results demonstrate the importance of YFV NS5 in overcoming the antiviral action of IFN-I and offer a unique example of a viral protein that is activated by the same host pathway that it inhibits. These studies were partly supported by NIAID grant U54AI057158 (to A.G.-S.) and by NIH fellowship FAI077333A (to M.L.-R.). We thank Richard Cadagan and Osman Lizardo for technical assistance, and Dr. Adriana Forero for editorial help. Confocal laser scanning microscopy was performed at ISMMS-Microscopy Shared Resource facility. application/pdf eng Cell Press Cell Host & Microbe. 2014, V. 16, n. 3, p. 314-327 https://doi.org/10.1016/j.chom.2014.07.015 info:eu-repo/grantAgreement/NIAID//U54AI057158/US/ info:eu-repo/grantAgreement/NIH//FAI077333A/US/ Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess Medicina Salud Microbiología Enfermedades infecciosas Medicine Health Microbiology Communicable diseases The Interferon Signaling Antagonist Function of Yellow Fever Virus NS5 Protein Is Activated by Type I Interferon info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Cell Host & Microbe 16 3 314 327 THUMBNAIL Laurent-chm_2014.pdf.jpg Laurent-chm_2014.pdf.jpg IM Thumbnail image/jpeg 4805 https://riubu.ubu.es/bitstream/10259/9529/4/Laurent-chm_2014.pdf.jpg 824b689159553929af608a8c94bf5a48 MD5 4 LICENSE license.txt license.txt text/plain; charset=utf-8 999 https://riubu.ubu.es/bitstream/10259/9529/3/license.txt b295bcbce42e2caabeb0c623d3860c06 MD5 3 CC-LICENSE license_rdf license_rdf application/rdf+xml; charset=utf-8 805 https://riubu.ubu.es/bitstream/10259/9529/2/license_rdf 4460e5956bc1d1639be9ae6146a50347 MD5 2 ORIGINAL Laurent-chm_2014.pdf Laurent-chm_2014.pdf application/pdf 1516211 https://riubu.ubu.es/bitstream/10259/9529/1/Laurent-chm_2014.pdf eed7968c457ba4e3f5af395970e45fc3 MD5 1 10259/9529 oai:riubu.ubu.es:10259/9529 2024-09-05 02:05:21.064 Repositorio Institucional de la Universidad de Burgos bubrep@ubu.es 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