2024-03-29T02:25:36Zhttps://riubu.ubu.es/oai/requestoai:riubu.ubu.es:10259/73162023-01-26T01:05:25Zcom_10259_3592com_10259_3591com_10259.4_106com_10259_2604com_10259_6168com_10259_5086com_10259_4313col_10259_3930col_10259_6169col_10259_4314
2023-01-25T12:38:53Z
urn:hdl:10259/7316
Lab-on-a-chip for the easy and visual detection of SARS-CoV-2 in saliva based on sensory polymers
Arnáiz Alonso, Ana
Guirado Moreno, José Carlos
Guembe García, Marta
Barros García, Rocío
Tamayo Ramos, Juan Antonio
Fernández Pampín, Natalia
García Pérez, José Miguel
Vallejos Calzada, Saúl
Peptide
Substrate
Fluorimetry
Immobilization
COVID-19
Paper-supported
Coating
Pandemics
The initial stages of the pandemic caused by SARS-CoV-2 showed that early detection of the virus in a simple way is the best tool until the development of vaccines. Many different tests are invasive or need the patient to cough up or even drag a sample of mucus from the throat area. Besides, the manufacturing time has proven insufficient in pandemic conditions since they were out of stock in many countries. Here we show a new method of manufacturing virus sensors and a proof of concept with SARS-CoV-2. We found that a fluorogenic peptide substrate of the main protease of the virus (Mpro) can be covalently immobilized in a polymer, with which a cellulose-based material can be coated. These sensory labels fluoresce with a single saliva sample of a positive COVID-19 patient. The results matched with that of the antigen tests in 22 of 26 studied cases (85% success rate).
2023-01-25T12:38:53Z
2023-01-25T12:38:53Z
2023-03
info:eu-repo/semantics/article
0925-4005
http://hdl.handle.net/10259/7316
10.1016/j.snb.2022.133165
eng
Sensors and Actuators B: Chemical. 2023, V. 379, 133165
https://doi.org/10.1016/j.snb.2022.133165
info:eu-repo/grantAgreement/Fundación Bancaria Caixa d'Estalvis i Pensions de Barcelona//LCF%2FPR%2FPR18%2F51130007/
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-113264RB-I00/ES/DISEÑO, SINTESIS Y CARACTERIZACION DE POLIMEROS INTELIGENTES. APLICACION DE SENSORES QUIMICOS POLIMERICOS EN CONTROL Y SEGURIDAD ALIMENTARIA Y EN EL AMBITO SANITARIO/
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
Atribución 4.0 Internacional
Elsevier