2024-03-28T15:29:16Zhttps://riubu.ubu.es/oai/requestoai:riubu.ubu.es:10259/61242022-11-15T13:25:37Zcom_10259_3844com_10259_5086com_10259_2604col_10259_3845
Olmo Alonso, Fabiola
Garoz Ruiz, Jesús
Colina Santamaría, Álvaro
Heras Vidaurre, Aránzazu
2021-11-08T12:56:54Z
2021-11-08T12:56:54Z
2020-09
1618-2642
http://hdl.handle.net/10259/6124
10.1007/s00216-020-02564-1
1618-2650
In this work, UV/Vis spectroelectrochemistry (SEC), in a thin-layer regime and parallel configuration, is selected to solve a
complex mixture that contains dopamine (DA), ascorbic acid (AA) and uric acid (UA). These molecules, like many other
biological compounds, are assuming a highly important place in analytical and biomedical fields due to the fundamental role
that they play in human metabolism. In addition, low or high levels of these compounds are associated with diseases such as
Parkinson’s disease. For this reason, the quantification of these biomolecules is becoming increasingly critical. However, some
drawbacks must be overcome, because the three molecules coexist in the human body, and the species are subject to mutual
interference. In fact, they are all oxidized at similar potentials, and their UV/Vis absorption bands overlap, greatly complicating
their quantification. For this reason, derivative SEC together with suitable chemometric tools such as PARAFAC are proposed to
solve this complex matrix. This technique allows us to separate the contribution of each of these molecules present in a sample
and to quantify all of them, achieving high resolution and reproducibility. Besides, detection limits at the micromolar level are
achieved for DA, AA and UA in mixture solutions. This work thus demonstrates the great potential for derivative potentiodynamic
SEC combined with the appropriate chemometric tools in solving complex mixtures, a field where SEC is still taking the
first steps.
Ministerio de Economía y Competitividad (Grants CTQ2017-83935-RAEI/
FEDER, UE), Junta de Castilla y León (Grant BU297P18) and
Ministerio de Ciencia, Innovación y Universidades (RED2018-102412-
T). F.O. is grateful for the contract funded by Junta de Castilla y León, the
European Social Fund and the Youth Employment Initiative. J.G.R.
thanks theMinisterio de Economía y Competitividad for his postdoctoral
contract (CTQ2017-83935-R AEI/FEDER, UE).
application/pdf
spa
Springer
Analytical and Bioanalytical Chemistry. 2020, V. 412, n. 24, p. 6329–6339
https://doi.org/10.1007/s00216-020-02564-1
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CTQ2017-83935-R/ES/ESPECTROELECTROQUIMICA RAMAN CUANTITATIVA
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/ RED2018-102412-T/ES/RED DE SENSORES Y BIOSENSORES ELECTROQUIMICOS
info:eu-repo/grantAgreement/Junta de Castilla y León//BU297P18
Spectroelectrochemistry
PARAFAC
Quantitative analysis
Derivative of absorbance with respect towavelength
Química analítica
Chemistry, Analytic
Derivative UV/Vis spectroelectrochemistry in a thin-layer regime: deconvolution and simultaneous quantification of ascorbic acid, dopamine and uric acid
info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
info:eu-repo/semantics/openAccess