2024-03-29T08:05:33Zhttps://riubu.ubu.es/oai/requestoai:riubu.ubu.es:10259/42582021-11-10T09:38:26Zcom_10259_4249com_10259_5086com_10259_2604col_10259_4250
Identification and quantification of carbamate pesticides in dried lime tree flowers by means of excitation-emission molecular fluorescence and parallel factor analysis when quenching effect exists
Rubio Martínez, Laura
Ortiz Fernández, Mª Cruz
Sarabia Peinador, Luis Antonio
Excitation-emission fluorescence
Parallel factor analysis
Carbamate pesticides
Quenching effect
Capability of detection
Dried lime tree flowers
Química analítica
Chemistry, Analytic
A non-separative, fast and inexpensive spectrofluorimetric method based on the second order calibration of excitation-emission fluorescence matrices (EEMs) was proposed for the determination of carbaryl, carbendazim and 1-naphthol in dried lime tree flowers. The trilinearity property of three-way data was used to handle the intrinsic fluorescence of lime flowers and the difference in the fluorescence intensity of each analyte. It also made possible to identify unequivocally each analyte. Trilinearity of the data tensor guarantees the uniqueness of the solution obtained through parallel factor analysis (PARAFAC), so the factors of the decomposition match up with the analytes. In addition, an experimental procedure was proposed to identify, with three-way data, the quenching effect produced by the fluorophores of the lime flowers. This procedure also enabled the selection of the adequate dilution of the lime flowers extract to minimize the quenching effect so the three analytes can be quantified. Finally, the analytes were determined using the standard addition method for a calibration whose standards were chosen with a D-optimal design.
The three analytes were unequivocally identified by the correlation between the pure spectra and the PARAFAC excitation and emission spectral loadings. The trueness was established by the accuracy line “calculated concentration versus added concentration” in all cases. Better decision limit values (CCα), in x0 = 0 with the probability of false positive fixed at 0.05, were obtained for the calibration performed in pure solvent: 2.97 μg L−1 for 1-naphthol, 3.74 μg L−1 for carbaryl and 23.25 μg L−1 for carbendazim. The CCα values for the second calibration carried out in matrix were 1.61, 4.34 and 51.75 μg L−1 respectively; while the values obtained considering only the pure samples as calibration set were: 2.65, 8.61 and 28.7 μg L−1, respectively.
theMinisteriodeEconomíayCompetitividad(CTQ2011-26022)
and JuntadeCastillayLeón(BU108A11-2)
2016-10-20T08:08:24Z
2016-10-20T08:08:24Z
2014-04
info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
0003-2670
http://hdl.handle.net/10259/4258
10.1016/j.aca.2014.02.008
eng
Analytica chimica acta. 2014, V. 820, p. 9–22
http://dx.doi.org/10.1016/j.aca.2014.02.008
info:eu-repo/grantAgreement/MINECO/CTQ2011-26022
info:eu-repo/grantAgreement/JCyL/BU108A11-2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
application/pdf
Elsevier
https://riubu.ubu.es/bitstream/10259/4258/7/Rubio-ACA_2014.pdf.jpg
Hispana
TEXT
http://creativecommons.org/licenses/by-nc-nd/4.0/
RIUBU. Repositorio Institucional de la Universidad de Burgos
http://hdl.handle.net/10259/4258