2024-03-28T09:10:41Zhttps://riubu.ubu.es/oai/requestoai:riubu.ubu.es:10259/48662023-03-31T12:32:27Zcom_10259_4759com_10259_2604com_10259_3924com_10259_5086col_10259_4760col_10259_3925
Colonization of electrospun polycaprolactone fibers by relevant pathogenic bacterial strains
Rumbo Lorenzo, Carlos
Tamayo Ramos, Juan Antonio
Caso, Federica
Rinaldi, Antonio
Romero Santacreu, Lorena
Quesada Pato, Roberto
Cuesta López, Santiago
bacterial attachment
biofilm
electrospun polycaprolactone
foodborne pathogens
microfibers
nosocomial pathogens
Química orgánica
Materiales
Chemistry, Organic
Materials
Electrospun biodegradable polymers have emerged as promising materials for their applications in several fields, including biomedicine and food industry. For this reason, the susceptibility of these materials to be colonized by different pathogens is a critical issue for public health, and their study can provide future knowledge to develop new strategies against bacterial infections. In this work, the ability of three pathogenic bacterial species (Pseudomonas aeruginosa, Acinetobacter baumannii, and Listeria monocytogenes) to adhere and form biofilm in electrospun polycaprolactone (PCL) microfibrous meshes was investigated. Bacterial attachment was analyzed in meshes with different microstructure, and comparisons with other materials (borosilicate glass and electrospun polylactic acid (PLA)) fibers were assessed. Analysis included colony forming unit (CFU) counts, scanning electron microscopy (SEM), and crystal violet (CV) staining. All the obtained data suggest that PCL meshes, regardless of their microstructure, are highly susceptible to be colonized by the pathogenic relevant bacteria used in this study, so a pretreatment or a functionalization with compounds that present some antimicrobial activity or antibiofilm properties is highly recommended before their application. Moreover, an experiment designed to simulate a chronic wound environment was used to demonstrate the ability of these meshes to detach biofilms from the substratum where they have developed, thus making them promising candidates to be used in wound cleaning and disinfection.
European Union’s H2020 research and innovation
programme under the Marie Skłodowska-Curie grant agreement
no. 691095 and Junta de Castilla y Leon-FEDER
(projects BU079U16 and BU092U16).
2018-07-25T10:25:06Z
2019-04-01T02:45:07Z
2018-04
info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
1944-8244
http://hdl.handle.net/10259/4866
10.1021/acsami.7b19440
eng
ACS Applied Materials & Interfaces. 2018, V. 10, n. 14, p. 11467–11473
https://doi.org/10.1021/acsami.7b19440
info:eu-repo/grantAgreement/EC/H2020/691095
info:eu-repo/grantAgreement/JCyL/BU079U16
info:eu-repo/grantAgreement/JCyL/BU092U16
info:eu-repo/semantics/openAccess
application/pdf
American Chemical Society
https://riubu.ubu.es/bitstream/10259/4866/4/Rumbo-AMI_2018.pdf.jpg
Hispana
TEXT
http://rightsstatements.org/vocab/CNE/1.0/
RIUBU. Repositorio Institucional de la Universidad de Burgos
http://hdl.handle.net/10259/4866