2024-03-28T11:07:16Zhttps://riubu.ubu.es/oai/requestoai:riubu.ubu.es:10259/57842023-03-31T11:47:19Zcom_10259_4759com_10259_2604col_10259_4760
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Schindler, Torben
author
González, Adrián
author
Boopathi, Ramachandran
author
Marty Roda, Marta
author
Romero Santacreu, Lorena
author
Wildes, Andew R.
author
Porcar, Lionel
author
Martel, Anne
author
Theodorakopoulos, Nikos
author
Cuesta López, Santiago
author
Angelov, Dimitar
author
Unruh, Tobias
author
Peyrard, Michel
author
2018-10
DNA is a flexible molecule, but the degree of its flexibility is subject to debate. The commonly-accepted
persistence length of lp ≈ 500Å is inconsistent with recent studies on short-chain DNA that show much greater
flexibility but do not probe its origin. We have performed x-ray and neutron small-angle scattering on a short
DNA sequence containing a strong nucleosome positioning element and analyzed the results using a modified
Kratky-Porod model to determine possible conformations. Our results support a hypothesis from Crick and Klug
in 1975 that some DNA sequences in solution can have sharp kinks, potentially resolving the discrepancy. Our
conclusions are supported by measurements on a radiation-damaged sample, where single-strand breaks lead to
increased flexibility and by an analysis of data from another sequence, which does not have kinks, but where our
method can detect a locally enhanced flexibility due to an AT domain.
2470-0045
http://hdl.handle.net/10259/5784
10.1103/PhysRevE.98.042417
2470-0053
Kinky DNA in solution: Small-angle-scattering study of a nucleosome positioning sequence