2024-03-29T04:37:22Zhttps://riubu.ubu.es/oai/requestoai:riubu.ubu.es:10259/43802022-04-29T12:02:47Zcom_10259_4376com_10259_5086com_10259_2604col_10259_4377
Acetylcholinesterase Inhibition-Based Biosensor for Aluminum(III) Chronoamperometric Determination in Aqueous Media
Artículo
Barquero Quirós, Miriam
Domínguez Renedo, Olga
Alonso Lomillo, Mª Asunción
Arcos Martínez, Julia
acetylcholinesterase
biosensor
aluminum
acetylthiocholine iodide
screen-printed electrodes
gold nanoparticles
Chemistry, Analytic
Química analítica
A novel amperometric biosensor for the determination of Al(III) based on the inhibition of the enzyme acetylcholinesterase has been developed. The immobilization of the enzyme was performed on screen-printed carbon electrodes modified with gold nanoparticles. The oxidation signal of acetylthiocholine iodide enzyme substrate was affected by the presence of Al(III) ions leading to a decrease in the amperometric current. The developed system has a detection limit of 2.1 ± 0.1 μM for Al(III). The reproducibility of the method is 8.1% (n = 4). Main interferences include Mo(VI), W(VI) and Hg(II) ions. The developed method was successfully applied to the determination of Al(III) in spiked tap water . The analysis of a certified standard reference material was also carried out. Both results agree with the certified values considering the respective associated uncertainties.
Research Vicerrectory of Costa Rica University (Project 804-B0-197), Junta de Castilla y León (BU212A12-2), Ministerio de Ciencia e Innovación (MICINN) and Fondo Europeo de Desarrollo Regional (FEDER) (INNPACTO SERIBIO 2011–2014) and TEC-2009/12029
2017-03-20T08:50:47Z
2017-03-20T08:50:47Z
2014-05
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://hdl.handle.net/10259/4380
eng
Sensors, 2014, V. 14, n. 5, p. 8203-8216
http://dx.doi.org/10.3390/s140508203
Attribution 3.0 Unported
http://creativecommons.org/licenses/by/3.0/
info:eu-repo/semantics/openAccess
application/pdf
MDPI