2024-03-28T16:12:44Zhttps://riubu.ubu.es/oai/requestoai:riubu.ubu.es:10259/48822021-11-10T09:38:24Zcom_10259_4365com_10259_5086com_10259_2604com_10259_4354com_10259_4883col_10259_4366col_10259_4355col_10259_4884
Fishing for G-Quadruplexes in solution with a perylene diimide derivative labeled with biotins
Busto Vázquez, Natalia
Calvo, Patricia
Santolaya, Javier
Leal Villalba, José María
Guédin, Aurore .
Barone, Giampaolo
Torroba Pérez, Tomás
Mergny, Jean-Louis
García Ruiz, Begoña
Química
Chemistry
A new fluorescent, non‐cytotoxic perylene diimide derivative with two biotins at the peri position, PDI2B, has been synthesized. This molecule is able to interact selectively with G‐quadruplexes with scarce or no affinity towards single‐ or double‐stranded DNA. These features have made it possible to design a simple, effective, safe, cheap, and selective method for fishing G‐quadruplex structures in solution by use of PDI2B and streptavidin coated magnetic beads. The new cyclic method reported leads to the recovery of more than 80 % of G‐quadruplex structures from solution, even in the presence of an excess of single‐stranded or duplex DNA as competitors. Moreover, PDI2B is a G4 ligand that can display higher thermal stabilization and greater affinity for 2‐ over 3‐tetrad quadruplexes, which constitutes a novel type of behavior.
“la Caixa” Foundation (LCF/PR/PR12/
11070003), MINECO (CTQ2014-58812-C2-2-R and CTQ2015-
71353-R, FEDER Funds), and Junta de Castilla y Lejn, Consejer
&a de Educacijn y Cultura y Fondo Social Europeo (Project
BU051U16), Spain, is gratefully acknowledged. N.B. is grateful
to Dr. Oscar Mendoza of the ARNA Laboratory for useful discussion
and also to the financial support of the Jos8 Castillejo
Program by the Spanish Ministry of Education, Culture and
Sports (JC2015-00403). J.L.M. acknowledges support from Conseil
Regional d’Aquitaine, Agence Nationale de la Recherche
(ANR Quarpdiem) and the Symbit program [CZ.02.1.01/0.0/0.0/
15_003/0000477] financed by the ERDF.
2018-08-24T11:28:04Z
2019-08-06T02:45:07Z
2018-08
info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
0947-6539
http://hdl.handle.net/10259/4882
10.1002/chem.201802365
eng
Chemistry-a european journal. 2018, V. 24, n. 44, p. 11292-11296
https://doi.org/10.1002/chem.201802365
info:eu-repo/grantAgreement/FundaciónLaCaixa/LCF-PR-PR12-11070003
info:eu-repo/grantAgreement/MINECO/CTQ2014-58812-C2-2-R
info:eu-repo/grantAgreement/MINECO/CTQ2015-71353-R
info:eu-repo/grantAgreement/JCyL/BU051U16
info:eu-repo/semantics/openAccess
application/pdf
Wiley-VCH Verlag