2024-03-29T12:04:45Zhttps://riubu.ubu.es/oai/requestoai:riubu.ubu.es:10259/51752023-03-31T12:26:50Zcom_10259_4759com_10259_2604col_10259_4760
Analysis of Polycaprolactone Microfibers as Biofilm Carriers for Biotechnologically Relevant Bacteria
Tamayo Ramos, Juan Antonio
Rumbo Lorenzo, Carlos
Caso, Federica
Rinaldi, Antonio
Garroni, Sebastiano
Notargiacomo, Andrea
Romero Santacreu, Lorena
Cuesta López, Santiago
electrospun polycaprolactone
microfibers
biofilm
bacterial attachment
biotechnology
Microbiología
Materiales
Microbiology
Materials
Polymeric electrospun fibers are becoming popular in microbial biotechnology because of their exceptional physicochemical characteristics, biodegradability, surface-to-volume ratio, and compatibility with biological systems, which give them a great potential as microbial supports to be used in production processes or environmental applications. In this work, we analyzed and compared the ability of Escherichia coli, Pseudomonas putida, Brevundimonas diminuta, and Sphingobium fuliginis to develop biofilms on different types of polycaprolactone (PCL) microfibers. These bacterial species are relevant in the production of biobased chemicals, enzymes, and proteins for therapeutic use and bioremediation. The obtained results demonstrated that all selected species were able to attach efficiently to the PCL microfibers. Also, the ability of pure cultures of S. fuliginis (former Flavobacterium sp. ATCC 27551, a very relevant strain in the bioremediation of organophosphorus compounds) to form dense biofilms was observed for the first time, opening the possibility of new applications for this microorganism. This material showed to have a high microbial loading capacity, regardless of the mesh density and fiber diameter. A comparative analysis between PCL and polylactic acid (PLA) electrospun microfibers indicated that both surfaces have a similar bacterial loading capacity, but the former material showed higher resistance to microbial degradation than PLA.
European Union’s H2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement no. 691095. The contracts of J.A.T.-R. and C.R. were supported by the grant nos. BU079U16 and BU092U16, that were co-financed by Junta de Castilla y León and the European Social Fund.
2019-11-12T11:34:01Z
2019-11-12T11:34:01Z
2018-09
info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
1944-8244
http://hdl.handle.net/10259/5175
10.1021/acsami.8b07245
1944-8252
eng
ACS Applied Materials and Interfaces. 2018, V. 10, n. 38, p. 32773-32781
https://doi.org/10.1021/acsami.8b07245
info:eu-repo/grantAgreement/JCyL/BU079U16
info:eu-repo/grantAgreement/JCyL/BU092U16
info:eu-repo/grantAgreement/EC/H2020/691095
info:eu-repo/semantics/openAccess
application/pdf
American Chemical Society