2024-03-29T02:28:47Zhttps://riubu.ubu.es/oai/requestoai:riubu.ubu.es:10259/61192022-11-15T13:51:45Zcom_10259_3844com_10259_5086com_10259_2604col_10259_3845
Dopamine-functionalized graphene oxide as a high-performance material for biosensing
Vulcano, Fabio
Kovtun, Alessandro
Bettini, Cristian
Xia, Zhenyuan
Liscio, Andrea
Terzi, Fabio
Heras Vidaurre, Aránzazu
Colina Santamaría, Álvaro
Zanfrognini, Barbara
Melucci, Manuela
graphene oxide
spectroelectrochemistry
chemical functionalization
1,2-dihydroxyphenyl moieties
NADH
We describe a nanocomposite material for the electrochemical detection of β-nicotinamide adenine dinucleotide (NADH), a coenzyme involved in redox reactions of all living cells and in the detection of many organic species by electrochemical biosensors. The composite is made of nanosheets of electrochemically exfoliated graphene oxide (EGO) covalently functionalized with dopamine (DP) molecules. The EGODP material finally obtained is rich of 1,2-dihydroxyphenyl moieties and is able to detect NADH at a particular low potential value with higher sensitivity with respect to pristine EGO.
To study the effectiveness of 1,2-dihydroxyphenyl moieties in inducing electrocatalytic oxidation of NADH, we combined standard voltammetric techniques with UV–Vis absorption spectroelectrochemistry, which allowed us to measure the variations in composition occurring at the electrode|solution interface, i.e. to measure the consumption rate of NADH.
Spectroelectrochemical tests performed by polarising the electrode at a fixed potential value were finally used to compare the performance of EGODP with both EGO and EGO-DP blend (MIX) for the detection of NADH. The covalently functionalized EGO (EGODP) shows sensitivity to NADH up to 300 M−1, around 180 % and 140 % better than either pristine EGO or MIX, respectively.
2021-11-08T12:02:02Z
2021-11-08T12:02:02Z
2021-11-08T12:02:02Z
2020-04
info:eu-repo/semantics/article
2053-1583
http://hdl.handle.net/10259/6119
10.1088/2053-1583/ab734f
2053-1583
spa
2D Materials. 2020, V. 7, n. 2, 024007
https://doi.org/10.1088/2053-1583/ab734f
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CTQ2017-83935-R/ES/ESPECTROELECTROQUIMICA RAMAN CUANTITATIVA
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/ RED2018-102412-T/ES/RED DE SENSORES Y BIOSENSORES ELECTROQUIMICOS
info:eu-repo/grantAgreement/Junta de Castilla y León//BU297P18//Fusión de técnicas espectroelectroquímicas avanzadas
http://creativecommons.org/licenses/by-nc-nd/3.0/
info:eu-repo/semantics/openAccess
Attribution-NonCommercial-NoDerivs 3.0 Unported
IOP Publishing