2024-03-29T07:55:09Zhttps://riubu.ubu.es/oai/requestoai:riubu.ubu.es:10259/52162023-03-31T12:25:53Zcom_10259_4759com_10259_2604col_10259_4760
Interaction analysis of commercial graphene oxide nanoparticles with unicellular systems and biomolecules
Domi, Brixhilda
Rumbo Lorenzo, Carlos
García Tojal, Javier
Elena Sima, Livia
Negroiu, Gabriela
Tamayo Ramos, Juan Antonio
graphene
unicellular organisms
toxicity
binding capacity
ATR-FTIR
TEM
ICP-MS
The ability of commercial monolayer graphene oxide (GO) and graphene oxide nanocolloids (GOC) to interact with different unicellular systems and biomolecules was studied by analyzing the response of human alveolar carcinoma epithelial cells, the yeast Saccharomyces cerevisiae and the bacteria Vibrio fischeri to the presence of different nanoparticle concentrations, and by studying the binding affinity of different microbial enzymes, like the α-l-rhamnosidase enzyme RhaB1 from the bacteria Lactobacillus plantarum and the AbG β-d-glucosidase from Agrobacterium sp. (strain ATCC 21400). An analysis of cytotoxicity on human epithelial cell line A549, S. cerevisiae (colony forming units, ROS induction, genotoxicity) and V. fischeri (luminescence inhibition) cells determined the potential of both nanoparticle types to damage the selected unicellular systems. Also, the protein binding affinity of the graphene derivatives at different oxidation levels was analyzed. The reported results highlight the variability that can exist in terms of toxicological potential and binding affinity depending on the target organism or protein and the selected nanomaterial.
2020-02-19T09:41:56Z
2020-02-19T09:41:56Z
2020-01
info:eu-repo/semantics/article
http://hdl.handle.net/10259/5216
10.3390/ijms21010205
1422-0067
eng
International Journal of Molecular Sciences. 2020, V. 21, n. 1, 205
https://doi.org/10.3390/ijms21010205
info:eu-repo/grantAgreement/EC/H2020/691095
info:eu-repo/grantAgreement/EC/H2020/721642
info:eu-repo/grantAgreement/EC/H2020/734873
info:eu-repo/grantAgreement/JCyL/BU079U16
info:eu-repo/grantAgreement/JCyL/BU291P18
info:eu-repo/grantAgreement/JCyL/BU022G18
info:eu-repo/grantAgreement/MINECO/CTQ2016-75023-C2-1-P
info:eu-repo/grantAgreement/MINECO/CTQ2015-70371-REDT
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
Atribución 4.0 Internacional
MDPI