2024-03-29T08:59:39Zhttps://riubu.ubu.es/oai/requestoai:riubu.ubu.es:10259/53702021-11-02T12:01:31Zcom_10259_3924com_10259_5086com_10259_2604col_10259_3925
Facilitated Anion Transport Induces Hyperpolarization of the Cell Membrane That Triggers Differentiation and Cell Death in Cancer Stem Cells
Soto Cerrato, Vanessa
Manuel Manresa, Pilar .
Hernando Santa Cruz, Elsa
Calabuig Fariñas, Silvia
Martínez Romero, Alicia
Fernández Dueñas, Víctor
Sahlholm, Kristoffer
Knöpfel, Thomas
García Valverde, María
Rodilla, Ananda M.
Jantus Lewintre, Eloisa
Farràs, Rosa
Ciruela, Francisco
Pérez Tomás, Ricardo
Quesada Pato, Roberto
Facilitated anion transport potentially represents a powerful tool to modulate various cellular functions. However, research into the biological effects of small molecule anionophores is still at an early stage. Here we have used two potent anionophore molecules inspired in the structure of marine metabolites tambjamines to gain insight into the effect induced by these compounds at the cellular level. We show how active anionophores, capable of facilitating the transmembrane transport of chloride and bicarbonate in model phospholipid liposomes, induce acidification of the cytosol and hyperpolarization of plasma cell membranes. We demonstrate how this combined effect can be used against cancer stem cells (CSCs). Hyperpolarization of cell membrane induces cell differentiation and loss of stemness of CSCs leading to effective elimination of this cancer cell subpopulation.
2020-07-10T09:36:52Z
2020-07-10T09:36:52Z
2015-12
info:eu-repo/semantics/article
0002-7863
http://hdl.handle.net/10259/5370
10.1021/jacs.5b09970
1520-5126
eng
Journal of the American Chemical Society. 2015, V. 137, n. 50, p. 15892-15898
https://doi.org/10.1021/jacs.5b09970
info:eu-repo/semantics/openAccess
American Chemical Society