2024-03-28T20:31:23Zhttps://riubu.ubu.es/oai/requestoai:riubu.ubu.es:10259/53482022-04-08T10:34:38Zcom_10259_4376com_10259_5086com_10259_2604col_10259_4377
Cunha Silva, Hugo
1
500
Pires, Filipa Velez
b46b5d5d-25f1-450d-b62a-2335acbdf741
500
Cabral, A. Cristina Dias
66ca5a93-94dd-401a-8c70-ba99cb0b13a2
500
Arcos Martínez, Julia
36
500
0000-0002-4932-6445
2020-06-18T08:42:57Z
2020-06-18T08:42:57Z
2019-12
0927-7765
http://hdl.handle.net/10259/5348
10.1016/j.colsurfb.2019.110490
Lactate oxidase (LOx), recognized to selectively catalyze the lactate oxidation in complex matrices, has been highlighted as preferable biorecognition element for the development of lactate biosensors. In a previous work, we have demonstrated that LOx crosslinking on a modified screen-printed electrode results in a dual range lactate biosensor, with one of the analysis linear range (4 to 50 mM) compatible with lactate sweat levels. It was advanced that such behavior results from an atypical substrate inhibition process. To understand such inhibition phenomena, this work relies in the study of LOx structure when submitted to increased substrate concentrations. The results found by fluorescence spectroscopy and dynamic light scattering of LOx solutions, evidenced conformational changes of the enzyme, occurring in presence of inhibitory substrate concentrations. Therefore, the inhibition behavior found at the biosensor, is an outcome of LOx structural alterations as result of a pH-dependent mechanism promoted at high substrate concentrations.
Spanish Ministry of Science and Innovation (MICINN), Ministry of Economy and Competitiveness (MINECO) and the European Regional Development Fund (FEDER) (TEC20013-40561-P and MUSSEL RTC-2015-4077-2). Hugo Cunha-Silva would like to acknowledge funding from the Spanish Ministry of Economy (BES-2014-068214)
application/pdf
eng
Elsevier
Colloids and Surfaces B: Biointerfaces. 2019, V. 184, 110490
https://doi.org/10.1016/j.colsurfb.2019.110490
info:eu-repo/grantAgreement/MINECO/MUSSEL RTC-2015-4077-2
info:eu-repo/grantAgreement/MICINN/TEC20013-40561-P
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Lactate oxidase inhibition
Amperometric biosensor
Lactic acid
Fluorescence spectrometry
Dynamic light scattering
Química analítica
Chemistry, Analytic
Inhibited enzymatic reaction of crosslinked lactate oxidase through a pH-dependent mechanism
info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
Colloids and Surfaces B: Biointerfaces
184
110490
TEXT
Cunha-csbb_2019.pdf.txt
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oai:riubu.ubu.es:10259/5348
2022-04-08 12:34:38.872
Repositorio Institucional de la Universidad de Burgos
bubrep@ubu.es
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