2024-03-29T00:27:00Zhttps://riubu.ubu.es/oai/requestoai:riubu.ubu.es:10259/55882023-03-31T12:19:22Zcom_10259_4759com_10259_2604col_10259_4760
Domi, Brixhilda
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0000-0001-7786-8615
Bhorkar, Kapil
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Rumbo Lorenzo, Carlos
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0000-0002-5038-0334
Sygellou, Labrini
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Yannopoulos, Spyros N.
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Barros García, Rocío
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0000-0002-0392-8504
Quesada Pato, Roberto
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0000-0003-2764-7157
Tamayo Ramos, Juan Antonio
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0000-0002-7071-002X
2021-01-12T11:18:06Z
2021-01-12T11:18:06Z
2021-01
http://hdl.handle.net/10259/5588
10.3390/ijms22020567
1422-0067
Boron nitride (BN) nanomaterials have been increasingly explored for potential applications in chemistry and biology fields (e.g., biomedical, pharmaceutical, and energy industries) due to their unique physico-chemical properties. However, their safe utilization requires a profound knowledge on their potential toxicological and environmental impact. To date, BN nanoparticles have been considered to have a high biocompatibility degree, but in some cases, contradictory results on their potential toxicity have been reported. Therefore, in the present study, we assessed two commercial 2D BN samples, namely BN-nanopowder (BN-PW) and BN-nanoplatelet (BN-PL), with the objective to identify whether distinct physico-chemical features may have an influence on the biological responses of exposed cellular models. Morphological, structural, and composition analyses showed that the most remarkable difference between both commercial samples was the diameter of their disk-like shape, which was of 200–300 nm for BN-PL and 100–150 nm for BN-PW. Their potential toxicity was investigated using adenocarcinomic human alveolar basal epithelial cells (A549 cells) and the unicellular fungus Saccharomycescerevisiae, as human and environmental eukaryotic models respectively, employing in vitro assays. In both cases, cellular viability assays and reactive oxygen species (ROS) determinations where performed. The impact of the selected nanomaterials in the viability of both unicellular models was very low, with only a slight reduction of S. cerevisiae colony forming units being observed after a long exposure period (24 h) to high concentrations (800 mg/L) of both nanomaterials. Similarly, BN-PW and BN-PL showed a low capacity to induce the formation of reactive oxygen species in the studied conditions. Even at the highest concentration and exposure times, no major cytotoxicity indicators were observed in human cells and yeast. The results obtained in the present study provide novel insights into the safety of 2D BN nanomaterials, indicating no significant differences in the toxicological potential of similar commercial products with a distinct lateral size, which showed to be safe products in the concentrations and exposure conditions tested.
application/pdf
eng
MDPI
International Journal of Molecular Sciences. 2021, V. 22, n. 2, 567
https://doi.org/10.3390/ijms22020567
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
2D boron nitride
eukaryotic model
nanotoxicity
cell viability
oxidative stress
Materia-Estructura
Materiales
Matter-Constitution
Materials
Assessment of Physico-Chemical and Toxicological Properties of Commercial 2D Boron Nitride Nanopowder and Nanoplatelets
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
International Journal of Molecular Sciences
22
2
567
TEXT
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2023-03-31 14:19:22.134
Repositorio Institucional de la Universidad de Burgos
bubrep@ubu.es
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