2024-03-29T12:11:14Zhttps://riubu.ubu.es/oai/requestoai:riubu.ubu.es:10259/66852022-06-24T11:00:39Zcom_10259_4313com_10259_5086com_10259_2604col_10259_4314
Arnáiz Alonso, Ana
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Guembe García, Marta
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600
Delgado-Pinar, Estefanía
fdc2fe9d-0c78-4795-b719-54954121831c
Valente, Artur J. M.
f63e6b05-eaee-4126-9d18-bca6f10d3b31
600
Ibeas Cortes, Saturnino
293
600
García Pérez, José Miguel
213
600
Vallejos Calzada, Saúl
567
600
2022-05-30T12:37:10Z
2022-05-30T12:37:10Z
2022-05
http://hdl.handle.net/10259/6685
10.1038/s41598-022-12848-4
2045-2322
We have faced the preparation of fully water-soluble fluorescent peptide substrate with long-term environmental stability (in solution more than 35 weeks) and, accordingly, with stable results in the use of this probe in determining the activity of enzymes. We have achieved this goal by preparing a co-polymer of the commercial N-vinyl-2-pyrrolidone (99.5% mol) and a fluorescent substrate for trypsin activity determination having a vinylic group (0.5%). The activity of trypsin has been measured in water solutions of this polymer over time, contrasted against the activity of both the commercial substrate Z-L-Arg-7-amido-4-methylcoumarin hydrochloride and its monomeric derivative, prepared ad-hoc. Initially, the activity of the sensory polymer was 74.53 ± 1.72 nmol/min/mg of enzyme, while that of the commercial substrate was 20.44 ± 0.65 nmol/min/mg of enzyme, the former maintained stable along weeks and the latter with a deep decay to zero in three weeks. The ‘protection’ effect exerted by the polymer chain has been studied by solvation studies by UV–Vis spectroscopy, steady-state & time resolved fluorescence, thermogravimetry and isothermal titration calorimetry.
"La Caixa" Foundation, under agreement LCF/PR/PR18/51130007. We also gratefully acknowledge the financial support provided by FEDER (Fondo Europeo de Desarrollo Regional) through Programa Operacional Factores de Competitividade (COMPETE), and the Spanish State Research Agency (PID2020-113264RB-I00/AEI/10.13039/501100011033). Centro de Química de Coimbra acknowledges Fundação para a Ciência e Tecnologia for financial support (Projects UIDB/00313/2020, UIDP/00313/2020). E.D.-P. thanks the “Concurso de Estímulo ao Emprego Científico” for the junior contract CEECIND/04136/2018.
application/pdf
eng
Springer Nature
Scientific Reports. 2022, V. 12, 8818
https://doi.org/10.1038/s41598-022-12848-4
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-113264RB-I00/ES/DISEÑO, SINTESIS Y CARACTERIZACION DE POLIMEROS INTELIGENTES. APLICACION DE SENSORES QUIMICOS POLIMERICOS EN CONTROL Y SEGURIDAD ALIMENTARIA Y EN EL AMBITO SANITARIO
info:eu-repo/grantAgreement/FCT//UIDB%2F00313%2F2020
info:eu-repo/grantAgreement/FCT//UIDP%2F00313%2F2020
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
Actuators
Sensors and biosensors
Química orgánica
Chemistry, Organic
The role of polymeric chains as a protective environment for improving the stability and efficiency of fluorogenic peptide substrates
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Scientific Reports
12
1
THUMBNAIL
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Arnaiz-sr_2022.pdf
Arnaiz-sr_2022.pdf
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10259/6685
oai:riubu.ubu.es:10259/6685
2022-06-24 13:00:39.084
Repositorio Institucional de la Universidad de Burgos
bubrep@ubu.es
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