RT info:eu-repo/semantics/article
T1 Barley seed coating with free and immobilized alkaline phosphatase to improve P uptake and plant growth
A1 Pilar Izquierdo, María Concepción
A1 Ortega Santamaría, Natividad
A1 Pérez Mateos, Manuel
A1 Busto Núñez, Mª Dolores
K1 Bioquímica
K1 Biochemistry
AB Coating barley seeds with free and immobilized alkaline phosphatase was investigated as a potential means toenhance plant utilization of accumulated soil phosphorus (P). Two coating techniques were studied: film-coatingand pelleting. The highest phosphatase activity retention in the coating layer, ranging from 0·48 to 0·67, wasobserved when seeds were film-coated with phosphatase–polyresorcinol complex (PPC). The germination ofseeds film-coated or pelleted with alkaline phosphatase ranged from 0·84 to 0·97 or 0·14 to 0·25, respectively.Low germination of the pelleted seeds was attributed to freezing the seeds in liquid nitrogen (N) for the layercoating formation. Pelleted seeds were not used in the remainder of the studies. Under pot culture conditions, anincrease in the soil inorganic P was detected when the seeds were film-coated with phosphatase. Moreover, thefilm-coating significantly increased the P uptake by plants (between 25 and 31% after 35 days after planting(DAP)). The present study showed that the seed film-coating with free and immobilized phosphatase increased thephosphatase activity in the rhizosphere and the P uptake by plants.
PB Cambridge University Press
SN 0021-8596
YR 2012
FD 2012-02
LK http://hdl.handle.net/10259.4/2518
UL http://hdl.handle.net/10259.4/2518
LA eng
DS Repositorio Institucional de la Universidad de Burgos
RD 27-abr-2024