RT info:eu-repo/semantics/article
T1 Arabidopsis Kunitz Trypsin Inhibitors in Defense Against Spider Mites
A1 Arnáiz Alonso, Ana
A1 Talavera Mateo, Lucía
A1 González Melendi, Pablo
A1 Martínez, Manuel
A1 Diaz, Isabel
A1 Santamaría Fernández, Mª Estrella
K1 Plant-herbivore interphase
K1 Tetranychus urticae
K1 Arabidopsis thaliana
K1 Serine protease inhibitors
K1 Cysteine protease inhibitors
K1 Spider mite digestion
K1 Biotecnología
K1 Biotechnology
AB Tetranychus urticae (two-spotted spider mite) is a striking example of polyphagy among herbivores with an extreme record of pesticide resistance and one of the most significant pests in agriculture. The T. urticae genome contains a large number of cysteine- and serine-proteases indicating their importance in the spider mite physiology. This work is focused on the potential role of the Kunitz trypsin inhibitor (KTI) family on plant defense responses against spider mites. The molecular characterization of two of these genes, AtKTI4 and AtKTI5, combined with feeding bioassays using T-DNA insertion lines for both genes was carried out. Spider mite performance assays showed that independent KTI silencing Arabidopsis lines conferred higher susceptibility to T. urticae than WT plants. Additionally, transient overexpression of these inhibitors in Nicotiana benthamiana demonstrated their ability to inhibit not only serine- but also cysteine-proteases, indicating the bifunctional inhibitory role against both types of enzymes. These inhibitory properties could be involved in the modulation of the proteases that participate in the hydrolysis of dietary proteins in the spider mite gut, as well as in other proteolytic processes.
PB Frontiers Media
YR 2018
FD 2018
LK http://hdl.handle.net/10259/10103
UL http://hdl.handle.net/10259/10103
LA eng
NO This work was supported by projects from Ministerio de Economía y Competitividad of Spain (projects BIO2014-53508-R, 618105-FACCE-Era Net Plus; BIO2017-83472-R).
DS Repositorio Institucional de la Universidad de Burgos
RD 31-ene-2025