RT info:eu-repo/semantics/article
T1 Isoflavone-rich extracts from okara using supercritical fluid extraction: Kinetic modeling and characterization
A1 Díaz de Cerio Alonso de Mezquía, Elixabet
A1 Barea Gómez, Pedro
A1 Melgosa Gómez, Rodrigo
A1 Illera, Alba Esther
A1 Sanz, María Teresa
A1 Beltrán Calvo, Sagrario
K1 Okara
K1 Soybean by-product
K1 Supercritical fluid extraction
K1 Isoflavones
K1 Tocopherols
K1 Biotecnología alimentaria
K1 Food-Biotechnology
K1 Alimentos-Composición
K1 Food-Composition
K1 Biotecnología
K1 Biotechnology
AB The lipid fraction and bioactive compounds from okara, a by-product of soybean processing, have been extractedby supercritical fluid extraction. The effect of operating parameters, pressure (20–40 MPa), temperature (40–80ºC), and cosolvent usage (0–10 %wt. ethanol), on the extraction kinetics has been investigated, satisfactorilycorrelating the experimental data to the Sovov´a’s model. Extraction yield by SFE ranged from 0.1014 g extract/ginsoluble solid (IS) at 20 MPa and 80 ºC to 0.1081 g extract/g IS at 30 MPa and 60 ºC (from 9.2 to 9.8 g extract/100 g okara, respectively), similar to Soxhlet extraction with n-hexane (9.2 ± 0.2 g extract/100 g). Pressurepositively affected the initial extraction rate, which also increased with temperature except for the lowestpressure of 20 MPa, exhibiting a typical cross-over behavior. The use of ethanol as cosolvent promoted the extraction of phenolic compounds and isoflavones, and was less important in the extraction of tocopherols. Extraction yield using ethanol increased up to 0.1100 g extract/g IS at 40 MPa and 40 ºC. As expected, the higher content of bioactive compounds in the sc-CO2 + ethanol extracts positively affected the antioxidant capacity, with the maximum found at 20 MPa, 40 ºC and 10 %wt. ethanol.
PB Elsevier
SN 0960-3085
YR 2024
FD 2024-12
LK http://hdl.handle.net/10259/10205
UL http://hdl.handle.net/10259/10205
LA eng
NO Financial support from Agencia Estatal de Investigacion ´ (AEI) and Ministerio de Ciencia e Innovacion ´ (MICINN) [grant number PID2022–136385OB-I00], from the AEI, MICINN, and NextGener ationEU [grant numbers TED2021–129311B-I00 and PDC2022–133443-I00] and from Junta de Castilla y Leon ´ (JCyL) and European Regional Development Fund (ERDF) [grant number BU027P23] is gratefully acknowledged. E Díaz de Cerio contract was funded by JCyL and ERDF through project BU050P20. P. Barea contract was funded by JCyL and the European Social Fund (ESF) by Orden EDU/ 1868/2022, de 19 de diciembre; R. Melgosa contract was funded by a Beatriz Galindo Research Fellowship [BG20/00182]; A.E. Illera contract was funded by JCyL and ERDF through project BU027P23.
DS Repositorio Institucional de la Universidad de Burgos
RD 22-feb-2025