RT info:eu-repo/semantics/article
T1 Optimization and stability of a reusable laccase-polymer hybrid film for the removal of bisphenol A in water
A1 Vallejo García, Jorge Lucas
A1 Vallejos Calzada, Saúl
A1 Trigo López, Miriam
A1 García Pérez, José Miguel
A1 Busto Núñez, Mª Dolores
K1 Acrylic polymer
K1 Biodegradation
K1 Bisphenol A
K1 Laccase
K1 Myceliophthora thermophila
K1 Trametes versicolor
K1 Materiales
K1 Materials
K1 Polímeros
K1 Polymers
AB Laccases are a group of versatile and green biocatalysts with high efficiency for the degradation of a wide variety of environmental pollutants. However, the feasibility of free laccase applications is hampered by enzyme inactivation due to exposure to denaturing conditions and difficult recovery, which limits reuse and continuous process design, resulting in high costs. Enzyme immobilization technology is a promising way of overcoming these drawbacks. In this work, laccases from two fungal sources, Myceliophthora thermophila and Trametes versicolor, were immobilized for the first time by covalent interaction (azo-linkages) on a film-shaped polyacrylic material. Optimal immobilization parameters were 10 U/mL enzyme load, 1 h and 25 ºC. The resultant immobilized laccases were characterized as a function of pH, temperature, and substrate concentrations (kinetic parameters) and compared with the free enzyme counterpart. The laccase-polymer hybrid (M. thermophila laccase) showed excellent operational and storage stability, retaining 89.8 % of the initial activity after 15 cycles of reuse and 10 days of storage at 4 ºC. Finally, the laccase-polymer films exhibited > 90 % depletion of bisphenol A in water by a dual adsorption-catalysis mechanism. The adsorption capacity ranged from 61.4 % to 30.5 %. GC-MS analysis revealed the degradation products 5,5′-bis-[1-(4-(4-hydroxyphenyl)-1-methyl-ethyl]-biphenyl-2,2′-diol and 3- or 2-methyl-2,3-ditrobenzofuran, as adsorbed compounds, as well as non-degraded bisphenol A. The significance of this research lies in the demonstrated catalytic efficiency of a new, reusable and cost-effective hybrid material for the removal of phenolic contaminants in water. The findings suggest that laccase-polymer hybrids could be a very useful tool for sustainable wastewater treatment.
PB Elsevier
SN 2352-1864
YR 2025
FD 2025-05
LK http://hdl.handle.net/10259/10329
UL http://hdl.handle.net/10259/10329
LA eng
NO We gratefully acknowledge the financial support provided by all funders. The financial support provided by Fondo Europeo de Desarrollo Regional-European Regional Development Fund (FEDER, ERDF) and Regional Government of Castilla y Le´ on -Consejería de Educaci´ on, Junta de Castilla y Le´ on - (BU025P23) is gratefully acknowledged. We also acknowledge grant PID2023–147301OB-I00 funded by MICIU/AEI /10.13039/501100011033 and FEDER, EU. This work was supported by the Regional Government of Castilla y Le´ on (Junta de Castilla y Le´ on) and the Ministry of Science and Innovation MICIN and the European Union NextGeneration EU PRTR. Author Saul Vallejos received grant BG22/00086 funded by the Spanish Ministerio de Universidades.
DS Repositorio Institucional de la Universidad de Burgos
RD 14-mar-2025