RT info:eu-repo/semantics/article
T1 Green extraction of isoflavones from okara using subcritical water: Kinetics, optimization, and comparison with other water-based sustainable methods
A1 Barea Gómez, Pedro
A1 Illera Gigante, Alba Ester
A1 Melgosa Gómez, Rodrigo
A1 Benito Román, Oscar
A1 Candela Gil, Helena
A1 Beltrán Calvo, Sagrario
A1 Sanz Díez, Mª Teresa
K1 Okara
K1 Isoflavones
K1 Subcritical water
K1 Kinetics
K1 Microwave-assisted extraction
K1 Ultrasound-assisted extraction
K1 Ingeniería Química
K1 Chemical engineering
K1 Biotecnología alimentaria
K1 Food-Biotechnology
K1 Bioquímica
K1 Biochemistry
AB Water has been explored as a green extraction agent for isoflavone extraction from okara. First, subcritical water (subW) was explored using two different okaras: (1) washed and dried (OKW) and (2) non-pretreated (OKC). Genistein family was the most abundant, with very low quantities in the glycitein family. Kinetic data revealed interconversion of malonyl-glycosides to β-glycosides in subW, with increasing degradation rate constants for all isoflavones with temperature. A maximum of 1229 μg isoflavone/g of dried okara was obtained at 120 °C after 30 min for OKC. Microwave (MAE) and ultrasound (UAE) technologies achieved 72.8 % and 75.4 %, of the yield of subW. Productivity at the maximum was of 41.0, 82.5 and 92.8 μg isoflavone/g dry okara min for subW, MAE, UAE. However, higher fraction of the more bioavailable forms β-glycoside + aglycone was obtained by subW.
PB Elsevier
SN 0308-8146
YR 2025
FD 2025-08
LK http://hdl.handle.net/10259/10445
UL http://hdl.handle.net/10259/10445
LA eng
NO This work was supported by the Agencia Estatal de Investigación [grant numbers PID2022-136385OB-I00, PDC2022–133443-I00, TED2021-129311B-I00], Junta de Castilla y León (JCyL), and European Regional Development Fund (ERDF) [grant number BU027P23]. A. E. Illera postdoctoral contract was funded by BU027P23. P. Barea and H. Candela pre-doctoral contracts are funded by JCyL and the European Social Fund (ESF) [ORDEN EDU/1868/2022, ORDEN EDU/1009/2024, respectively]. The R Melgosa contract was funded by a Beatriz Galindo Research Fellowship [BG20/00182].
DS Repositorio Institucional de la Universidad de Burgos
RD 09-may-2025