RT info:eu-repo/semantics/article T1 Isolation, Optimization, and Structural Characterization of Melanoidins Obtained from Bread Industry By-products as Bioactive Compounds A1 Salazar Mardones, Gonzalo A1 Cavia Saiz, Mónica A1 Temiño, Virginia A1 Muñiz Rodríguez, Pilar A1 García Tojal, Javier A1 Gerardi, Gisela K1 Melanoidins K1 FTIR K1 Ultrafiltration K1 Bakery by-products K1 Bread crust K1 Scavenger activity K1 Alimentos-Análisis K1 Food-Analysis K1 Alimentos-Composición K1 Food-Composition AB Melanoidins are bioactive compounds that have an impact on food properties and have effects on health. Due to the high content of melanoidins in bread crust, it can be used as a source of these compounds. Their recovery from bakery by-products can reduce environmental pollution and provide added value. The aim of this study was to evaluate the efficiency of ultrafiltration to obtain melanoidin extracts from crust of common and soft bread obtained as industrial by-products and to compare their structure and antioxidant and chelating activity. The high-molecular-weight (HMW > 10 kDa) melanoidin extract was obtained by ultrafiltration conditions optimized to ensure minimum fouling and to obtain a melanoidin extract with a minimum content of low-molecular-weight (LMW) compounds that could be toxic. FTIR spectroscopy revealed significant similarities between both melanoidin samples, but differences were observed with their LMW fractions. Melanoidins, which are melanoproteins, exhibited higher carbohydrate levels in the extract from soft bread, whereas the extract from common bread contained more proteins and polyphenolic compounds. Despite having a lower browning index and K420, the melanoidin from soft bread exhibited higher antiradical and chelating properties. In conclusion, this study provides our understanding of the structural and functional aspects of bread melanoidins. PB Springer SN 1935-5130 YR 2025 FD 2025-04 LK https://hdl.handle.net/10259/11138 UL https://hdl.handle.net/10259/11138 LA eng NO Open access funding provided by FEDER European Funds and the Junta de Castilla y León under the Research and Innovation Strategy for Smart Specialization (RIS3) of Castilla y León 2021-2027. The authors acknowledge financial support of Ministry of Science and Innovation, Spanish State Research Agency and European Regional Development Fund (TED201 - 132195B-I00) DS Repositorio Institucional de la Universidad de Burgos RD 20-abr-2026