RT info:eu-repo/semantics/article
T1 Genotyping, virulence genes and antimicrobial resistance of Campylobacter spp.isolated during two seasonal periods in Spanish poultry farms
A1 García Sánchez, Lourdes
A1 Melero Gil, Beatriz
A1 Diez Mate, Ana Mª
A1 Jaime Moreno, Isabel
A1 Canepa Oneto, Antonio Jesús
A1 Rovira Carballido, Jordi
K1 Poultry farms
K1 Prevalence
K1 PFGE
K1 Virulence genes
K1 Antibiotic resistance
K1 Spain
K1 Salud pública veterinaria
K1 Veterinary public health
K1 Epidemiología
K1 Epidemiology
AB Campylobacter spp. are the leading causes of bacterial human gastroenteritis worldwide; being poultry farms the main source of infections. In order to obtain information on prevalence and diversity of Campylobacter-infected flocks in the North of Spain, fourteen farms were studied between autumn and spring in 2014 and 2015, respectively. Moreover, virulence genes involved in pathogenicity and antimicrobial resistance were investigated. A survey about preventive hygiene practices at farms was performed to determine the risky practices that could contribute to the presence of Campylobacter in this step of the poultry food chain. Testing the presence of Campylobacter spp. showed 43 % of the farms were positive during autumn, whereas only 31 % were positive in spring. A very high prevalence within-flock was observed (43.1 % to 88.6 %) and C. jejuni was the most prevalent species in both periods. Genotyping by pulsed field gel electrophoresis (PFGE) showed a high heterogeneity among farms (309 isolates clustered into 21 pulsotypes). Virulence genes were present in all C. jejuni isolates while cdtA and cdtC were absent in C. coli. On the contrary, the latter showed higher antimicrobial resistance than C. jejuni. This study suggests that environment might be one of the main sources for Campylobacter transmission, as water supply seemed to be a clear cause of the contamination in a specific farm. However, in other farms other environmental factors contributed to the contamination, confirming the multifactorial origin of Campylobacter colonization in broilers. Therefore, biosecurity measures in farms are crucial to reduce Campylobacter contamination, which may have important implications for human and animal health.
PB Elsevier
SN 0167-5877
YR 2020
FD 2020-03
LK https://hdl.handle.net/10259/11265
UL https://hdl.handle.net/10259/11265
LA eng
NO This work was supported by a project founded by “Ministerio de Economía y Competitividad” from the Spanish Government (AGL 2013-47694-R) and by “Junta de Castilla y Leon” (BU159U14). The PhD grant of Lourdes García Sanchez is funded by University of Burgos, Spain (12-PR00).
DS Repositorio Institucional de la Universidad de Burgos
RD 21-abr-2026