RT info:eu-repo/semantics/article T1 High-Pressure Processing for Cold Brew Coffee: Safety and Quality Assessment under Refrigerated and Ambient Storage A1 Polanco-Estibález, Berta A1 García-Santa-Cruz, Rodrigo A1 Queirós, Rui P. A1 Serment-Moreno, Vinicio A1 González Angulo, Mario A1 Tonello Samson, Carole A1 Rivero Pérez, Maria Dolores K1 High-pressure processing K1 Cold brew coffee K1 Escherichia coli O157:H7 K1 Listeria monocytogenes K1 Salmonella enterica K1 Tecnología de los alimentos K1 Industria alimentaria-Salubridad K1 Food industry and trade-Sanitation AB Cold brew coffee (CBC) has gained in popularity due to its distinct sensory experience. However, CBC can pose a risk for bacterial pathogens if not stored properly. High-Pressure Processing (HPP) is a nonthermal technology that can improve the safety of CBC while maintaining its quality. In this study, CBC made from ground roasted coffee grains was processed at 600 MPa for 3 min and stored at 4 or 23 °C for 90 days. The microbiological quality indicators remained stable throughout the study period. Physicochemical and quality parameters, such as pH, total dissolved solids, titratable acidity, color, total phenolic compounds and antioxidant activity, were not significantly affected by HPP. Both unprocessed and HPP CBC samples showed changes in pH, titratable acidity and color stability after 60 days at 23 °C. Unprocessed CBC samples spiked with Escherichia coli O157:H7, Listeria monocytogenes and Salmonella enterica showed decreased counts, but the pathogens were still detectable after 60 days at 4 °C and after 90 days at 23 °C. HPP achieved a >6-log10 reduction in the species tested, with non-detectable levels for at least 90 days at both storage temperatures. These findings suggest that HPP can effectively control vegetative pathogens and spoilage microorganisms in CBC while preserving its quality attributes. PB MDPI SN 2304-8158 YR 2023 FD 2023-12 LK https://hdl.handle.net/10259/11286 UL https://hdl.handle.net/10259/11286 LA eng NO This research was funded by Instituto para la Competitividad Empresarial (ICE) of Junta de Castilla y León through the R&D project 10/18/BU/0016, and co-funded by the European Regional Development Fund (ERDF)—A way to make Europe. DS Repositorio Institucional de la Universidad de Burgos RD 28-abr-2026