RT info:eu-repo/semantics/article T1 Boosting betalain bioaccessibility from prickly pear peel through pulsed electric fields (PEF) and pressurized liquid extraction (PLE) A1 Calleja Gomez, Mara A1 Pallarés, Noelia A1 Gaona Ruiz, María A1 Barba, Francisco J. A1 Carrillo Pérez, Celia K1 Bioaccessibility K1 In vitro digestion K1 Pigments K1 Revalorization K1 Green technologies K1 Bioquímica K1 Biochemistry AB Betalains are bioactive pigments with antioxidant and health-promoting potential, abundant in prickly pear (Opuntia ficus-indica) peel, an underutilized by-product. This study evaluated the efficiency of pulsed electric fields (PEF) and pressurized liquid extraction (PLE) for betalain recovery and their impact on compound bioaccessibility after in vitro digestion. Freeze-dried peels were treated with PEF (2.5 kV/cm, 50 kJ/kg) and PLE (50 °C, 1500 psi, pH 7), and compared to conventional aqueous maceration. Betalains were quantified by UV/Vis and characterized by LC-Q-TOF-MS/MS, while bioaccessibility was assessed using the INFOGEST protocol. PLE increased total betalain yield by 36.3%, whereas PEF enhanced bioaccessibility despite a lower initial concentration. Both techniques preserved 21–22 betalain derivatives, including indicaxanthin and betanin, showing high stability during digestion. These results highlight the potential of PEF and PLE as sustainable technologies for efficient extraction and stabilization of betalains, supporting circular bioeconomy strategies through the valorization of prickly pear peels. PB Elsevier SN 0308-8146 YR 2026 FD 2026-07 LK https://hdl.handle.net/10259/11659 UL https://hdl.handle.net/10259/11659 LA spa NO This research is part of the project PID2021-123628OB-C42 funded by MICIU/AEI/10.13039/501100011033/FEDER, UE. Mara Calleja-Gómez is a beneficiary of the predoctoral grant from the Conselleria d'Educació, Universitats i Ocupació of Generalitat Valenciana (CIACIF/2022/391). DS Repositorio Institucional de la Universidad de Burgos RD 05-oct-2026