RT info:eu-repo/semantics/article
T1 Pd(II)/1,10-phenanthroline complexes bearing arene ligands: On the role of N- vs O-coordination to tune their cellular activity and binding ability towards DNA and RNA
A1 Binacchi, Francesca
A1 Cirri, Damiano
A1 Bimbi, Eleonora
A1 Busto Vázquez, Natalia
A1 Pratesi, Alessandro
A1 Biver, Tarita
K1 Metal complexes
K1 Pd(II) cytotoxyc compounds
K1 ROS production
K1 Cancer cell selectivity
K1 Coordination
K1 DNA cleavage
K1 Bioquímica-Investigación
K1 Biochemistry-Research
AB Three Pd(II)-based complexes of 1,10-phenanthroline and N- or O-coordinating ligands have been synthesised and tested with different relevant biosubstrates like double-stranded DNA, double and triple helix of RNA, DNA G-quadruplexes of different conformations and bovine serum albumin. Here a correlation between N- vs O-coordinating elements and binding mechanism emerged, where the N-coordinating ligands proved to be the most promising. These outcomes were confirmed also in the cellular experiments. The Pd(II) complex with naphthalene-1,8-diamine is the one that is able to be carried by BSA, to strongly bind nucleic acids, to produce reactive oxygen species (ROS) and to show the best cellular performances (poorly toxic towards healthy cells and highly toxic against the cisplatin-resistant cancer cell line). On the opposite, the complex with benzene-1,2- diolate may be sequestered by BSA, weakly binds nucleic acids, does not produce ROS and shows poor cellular activity. The complex with benzene-1,2-diamine stays in between. Other mechanistic details are discussed which show that the biophysical behaviour is the sum of the contribution of aromaticity, charge and N- or O-coordination.
PB Elsevier
SN 0162-0134
YR 2025
FD 2025-01
LK https://hdl.handle.net/10259/11879
UL https://hdl.handle.net/10259/11879
LA eng
NO This contribution is part of the work from COST Action CA18202, NECTAR Network for Equilibria and Chemical Thermodynamics Advanced Research, supported by COST (European Cooperation in Science and Technology).NB acknowledges Ministerio de Ciencia e Innovaci´on of Spain (PID2022-142318NB-I00) for financial support.AP gratefully acknowledges funding by the University of Pisa under the “PRA–Progetti di Ricerca di Ateneo” (Institutional Research Grants), project no. PRA_2022–2023_12 “New challenges of transition metal and lanthanide complexes in the perspective of green chemistry”. The CIRCC (Interuniversity Consortium for Chemical Reactivity and Catalysis) is also acknowledged.
DS Repositorio Institucional de la Universidad de Burgos
RD 27-jun-2026