RT info:eu-repo/semantics/article
T1 Cholesterol Oxidases Act as Signaling Proteins for the Biosynthesis of the Polyene Macrolide Pimaricin
A1 Mendes, Marta V.
A1 Recio, Eliseo
A1 Antón Fidalgo, Nuria
A1 Guerra, Susana M.
A1 Santos-Aberturas, Javier
A1 Martín, Juan F.
A1 Aparicio, Jesús F.
K1 Cholesterol oxidases
K1 Signaling proteins
K1 Polyene Macrolide Pimaricin
K1 Genética bacteriana
K1 Bacterial genetics
K1 Antibióticos
K1 Antibiotics
AB The gene cluster responsible for pimaricin biosynthesis in Streptomyces natalensis contains a cholesterol oxidase-encoding gene (pimE) surrounded by genes involved in pimaricin production. Gene-inactivation and -complementation experiments revealed that pimE encodes a functional cholesterol oxidase and, surprisingly, that it is also involved in pimaricin biosynthesis. This extracellular enzyme was purified from S. natalensis culture broths to homogeneity, and it was shown to restore pimaricin production when added to the mutant culture broths. Other cholesterol oxidases also triggered pimaricin production, suggesting that these enzymes could act as signaling proteins for polyene biosynthesis. This finding constitutes the description of a cholesterol oxidase gene with an involvement in antibiotic biosynthesis, and it broadens the scope of the biological functions for this type of oxidase.
PB Cell press
SN 1074-5521
YR 2007
FD 2007-03
LK https://hdl.handle.net/10259/12150
UL https://hdl.handle.net/10259/12150
LA eng
DS Repositorio Institucional de la Universidad de Burgos
RD 03-oct-2026