RT info:eu-repo/semantics/article
T1 The two-component phoR-phoP system of Streptomyces natalensis: Inactivation or deletion of phoP reduces the negative phosphate regulation of pimaricin biosynthesis
A1 Mendes, Marta V.
A1 Tunca, Sedef
A1 Antón Fidalgo, Nuria
A1 Recio, Eliseo
A1 Sola-Landa, Alberto
A1 Aparicio, Jesús F.
A1 Martín, Juan F.
K1 Pimaricin
K1 Polyene
K1 Polyketide synthase
K1 Phosphate regulation
K1 PHO boxes
K1 PhoP
K1 DNA binding
K1 Genética bacteriana
K1 Bacterial genetics
K1 Antibióticos
K1 Antibiotics
AB The biosynthesis of the antifungal pimaricin in Streptomyces natalensis is very sensitive to phosphate regulation. Concentrations of inorganic phosphate above 1 mM drastically reduced pimaricin production. At 10 mM phosphate, expression of all the pimaricin biosynthesis (pim) genes including the pathway-specific positive regulator pimR is fully repressed. The phoU-phoR-phoP cluster of S. natalensis encoding two-component Pho system was cloned and sequenced. Binding of the response regulator PhoP to the consensus PHO boxes in the phoU-phoRP intergenic promoter region was observed. A phoP-disrupted mutant and a phoR-phoP deletion mutant were obtained. Production of pimaricin in these two mutants increased up to 80% in complex yeast extract–malt extract (YEME) or NBG media and showed reduced sensitivity to phosphate control. Four of the pim genes, pimS1, pimS4, pimC and pimG showed increased expression in the phoP-disrupted mutant. However, no consensus PHO boxes were found in the promoter regions of any of the pim genes, suggesting that phosphate control of these genes is mediated indirectly by PhoR-PhoP involving modification of pathway-specific regulators.
PB Elsevier
SN 1096-7176
YR 2007
FD 2007
LK https://hdl.handle.net/10259/12152
UL https://hdl.handle.net/10259/12152
LA eng
DS Repositorio Institucional de la Universidad de Burgos
RD 26-sep-2026