RT info:eu-repo/semantics/conferenceObject T1 Biorefinery concept to valorize olive leaves and twigs generated in the olive mill by mild subcritical water A1 Illera Gigante, Alba Ester A1 Sanz Díez, Mª Teresa A1 Benito Román, Oscar A1 Melgosa Gómez, Rodrigo A1 Lionetti, Vincenzo A1 Caminada, Giulia A1 D'Avanzo, Nicola A1 Gentile, Mariagrazia A1 Beltrán Calvo, Sagrario K1 Olive leaves K1 Subcritical water K1 Phenolic compounds AB This work is part of a wider project (OLinWASTE) aimed at developing an innovative zeroemission biorefinery transforming the olive mill waste (OMW), including pomace, stones, andleaves into valuable resources. The olive leaves at twigs (OL) were provided by the Italian olivemill Agrolio (Coratina cultivar). OL contain 44 ± 2 % (wt.) moisture and has the followingmonosaccharide composition: xylose (43 ± 4), followed by arabinose (20 ± 5) and galacturonicacid (11 ± 3), with lower amounts of other monosaccharides and bioactive compounds. Theextraction of phenolic compounds from wet, ground OL was studied using subcritical water (SW)treatment under mild conditions: 120, 130 and 140 ºC for 30 min, in a batch reactor of 0.5 L. Thepercentage of total soluble solids increased with temperature with values of 29.8 ± 0.5, 34.2 ± 0.7and 37.2 ± 0.9, respectively. The extracts presented an interesting phenolic profile and antioxidantcapacity, with oleuropein, hydroxytirosol, and luteolin-7-O-glucoside being the major identifiedphenolic compounds, accounting for nearly 90 % of the phenolic content. Luteolin-7-O-glucosideand oleuropein content decreased with temperature, while hydroxytirosol increased due to thermaldegradation of oleuropein, releasing hydroxytyrosol, a potent antioxidant, reaching values of 469± 28 mg per 100 g of total solids in the extract, from a total of 636 ± 34 phenolic compounds inthe total solid extract at 140 ºC. These results show that valorization of OMW by SW offerssignificant potential for producing valuable bioactive compounds. YR 2025 FD 2025 LK https://hdl.handle.net/10259/12217 UL https://hdl.handle.net/10259/12217 LA eng NO Póster presentado en: XVI International Scientific Agriculture Symposium "Agrosym 2025", Jahorina, Bosnia and Herzegovina, October 2-5, 2025 NO This work was supported by European Commission Program HORIZONCL6-2024-ZEROPOLLUTION-02- Innovative technologies for zero pollution, zero-waste biorefineries [project number 101181402]. A.E. Illera postdoctoral contract was funded by the BU027P23 project of the JCyL. O. Benito-Román postdoctoral contract was funded by a “Ramón y Cajal” contract of the “Ministerio de Ciencia, Innovación y Universidades” (Programa PEICTI 2024-2027). DS Repositorio Institucional de la Universidad de Burgos RD 03-oct-2026