RT info:eu-repo/semantics/conferenceObject
T1 SWI/SNF alterations in cancer: molecular targets and therapeutic implications
A1 Monteverde, Beatriz
A1 Moreno, Thaidy
A1 Fuente Vivas, Dalia de la
A1 Revilla, Carlos
A1 Casar, Berta
A1 Crespo, Piero
A1 Varela, Ignacio
K1 Cromatina
K1 Chromatin
K1 Genética médica
K1 Medical genetics
AB Background:SWI/SNF chromatin remodelling complex is altered in nearly 20% of all human tumor types, which places it as the most broadlymutated molecular system in human cancer, just after TP53 [1, 2]. Nevertheless, the molecular mechanisms through which thesealterations foster cancer development remain to be fully understood. In addition, any potential susceptibility associated withSWI/SNF alterations might be exploited for the treatment of patients affected by different malignancies.Methodology:We have generated stably-transduced cell lines for a doxycycline-inducible vector that directs the expression of different shRNAstargeting ARID1A, ARID1B or ARID2 in different human cancer cell lines. We have studied the transcriptional alterations resultedfrom the deficiency of these subunits in different cellular contexts by RNA-Seq, as well as the changes in chromatin accessibilityby ATAC-Seq. Additionally, we have studied the sensitivity of these cell lines to different antitumoral treatments.Results:We have identified molecular pathways differently expressed in the SWI/SNF-deficient cell lines that might explain somecharacteristics of the behaviour of these tumor cells. Thus, we have seen an overexpression of genes involved in ROS production,as well as genes encoding metallopeptidases, and a downregulation of genes related to cellular adhesion or tumor suppression.Finally, we have detected different sensitivities in the ARID-deficient cells to some treatments, like cis-platin, and PARP or EGFRinhibitors.
YR 2019
FD 2019
LK https://hdl.handle.net/10259/12242
UL https://hdl.handle.net/10259/12242
LA eng
NO Póster presentado en: 42 Congress SEBBM, Madrid, 16-19 july 2019
DS Repositorio Institucional de la Universidad de Burgos
RD 08-oct-2026