RT info:eu-repo/semantics/bachelorThesis
T1 Determinación analítica de fármacos con propiedades antiepilépticas
A1 Iglesias García, Ángela
A2 Universidad de Burgos. Departamento de Química
K1 Química analítica
K1 Chemistry, Analytic
AB The necessity of disposable biosensors for simple, rapid and inexpensiveanalysis in fields such as clinical, environmental or industrial has been highlightedover the past decade. In this way, screen-printed electrodes (SPEs) have been shownas inexpensive and reproducible devices for mass production of miniaturizedbiosensors [1-4]. These transducers, building by sequential layer deposition on thesurface of ceramic or plastic substrates and curing steps, have been conventionallylinked to the sensing element by adsorption, cross-linking, electropolymerization orcovalent bonding. Bioelements are commonly immobilized after the printing andfiring processes, because of the high temperatures reached during the curing step [5].The immobilization procedure requires maintaining the initial properties of theenzyme intact. Thus, successful developments of biosensors largely rely on the costand stability of the sensing elements [3].Even if the above-mentioned immobilization procedures are efficient, theyimply additional steps after fabrication of the screen-printed carbon electrodes(SPCEs), which extends the whole biosensor manufacturing. Screen-printingtechniques also offer another attractive immobilization procedure consisting ofprinting the biological material. Enzymes, which are proteins able to catalyse specificchemical reactions in vivo, are by far the most commonly employed bioelements [1].Enzymes can be integrated into the ink to form the sensing paste, which can bescreen-printed resulting in biosensors fabricated by only one technology [6-8].Undoubtedly, this immobilization procedure, which is known as automatedimmobilization, is particularly interesting for mass production of disposablebiosensors.This work presents a simple way for preparing SPCEs modified withHorseradish peroxidase (HRP) for the determination of Levetiracetam (LEV). Thissecond-generation antiepileptic drug (AEDs) has been previously determined using aSPCE-biosensor based on the immobilization of Horseradish peroxidase (HRP) bypyrrole electropolymerization [9] and covalent bonding [10] The screen-printing ofHRP-containing ink (SPCHRPEs) offers a higher rapidity and simplicity in themanufacturing process of biosensors for LEV determination.
LK http://hdl.handle.net/10259/3818
UL http://hdl.handle.net/10259/3818
LA spa
DS Repositorio Institucional de la Universidad de Burgos
RD 04-dic-2024