RT info:eu-repo/semantics/article
T1 Glycerolysis of sardine oil catalyzed by a water dependent lipase in different tert-alcohols as reaction medium
T2 Glicerolisis de aceite de sardina catalizada por una lipasa dependiente de agua en diferentes
tert-alcoholes como medio de reacción
A1 García Solaesa, Ángela
A1 Sanz Díez, Mª Teresa
A1 Melgosa Gómez, Rodrigo
A1 Bucio López, Silvia Liliana
A1 Beltrán Calvo, Sagrario
K1 Fish oil
K1 Glycerolysis
K1 Lipase
K1 Monoacylglycerol
K1 Rhizomucor miehei
K1 Tert-alcohols
K1 Aceite de pescado
K1 Glicerolisis
K1 Lipasa
K1 Monoacilglicéridos
K1 Tert-alcoholes
K1 Ingeniería química
K1 Chemical engineering
AB The production of monoacylglycerol rich in polyunsaturated fatty acids (PUFA) via enzymaticglycerolysis of sardine oil in a homogeneous system was evaluated. Reactions were conducted in two differenttert-alcohols. Based on the phase equilibrium data, the amount of solvent added to create a homogeneoussystem has been calculated and optimized. The immobilized lipase used in this work was Lipozyme RM IMfrom Rhizomucor miehei, a water dependent lipase. The amount of water added as well as other reaction parameterswere studied to evaluate the optimum conditions for monoacylglycerol obtencion. An initial reactant moleratio glycerol to sardine oil 3:1, 12 wt% of water based on glycerol content and 10 wt% of lipase loading (basedon weight of reactants), achieved a MAG yield of around 70%, with nearly 28 wt% PUFA, with low free fattyacid content (lower than 18 wt%).
PB Consejo Superior de Investigaciones Científicas
SN 0017-3495
YR 2015
FD 2015-10
LK http://hdl.handle.net/10259/4246
UL http://hdl.handle.net/10259/4246
LA eng
NO Spanish Governmentthrough MINECO and the European RegionalDevelopment Fund (ERDF) for financial supportto the project CTQ2012-39131-C02-01. They wishto thank Novozymes A/S for kindly supplying theenzymes. RM acknowledges MINECO for an FPIgrant (reference BES-2013-063937).
DS Repositorio Institucional de la Universidad de Burgos
RD 24-dic-2024