RT info:eu-repo/semantics/article
T1 Robustness testing in the determination of seven drugs in animal muscle by liquid chromatography–tandem mass spectrometry
A1 Oca Casado, Mª Leticia
A1 Rubio Martínez, Laura
A1 Ortiz Fernández, Mª Cruz
A1 Sarabia Peinador, Luis Antonio
A1 García, Inmaculada .
K1 Robustness
K1 Tranquilizer
K1 Liquid chromatography–tandem
K1 mass spectrometry
K1 Commission Decision No 2002/657/EC
K1 Lenth's method
K1 Bayesian analysis
K1 Química analítica
K1 Chemistry, Analytic
AB In this work, the robustness of the sample preparation procedure for the determination of six tranquilizers (xylazine, azaperone, propionylpromazine, chlorpromazine, haloperidol, and azaperol) and a beta-blocker (carazolol) in animal muscle by LC/MS–MS was assessed through the experimental design methodology. A 2III7 − 4 fractional factorial design was performed to evaluate the influence of seven variables on the final concentration of the seven drugs in the samples, in accordance with what is laid down in Commission Decision No 2002/657/EC. The variation considered for each of those seven factors is likely to happen when preparing the samples, being the values chosen as level − 1, the nominal operating conditions. The results of the experimentation were evaluated from different statistical strategies, such as hypothesis testing using an external variance previously estimated, Lenth's method, and Bayesian analysis. Both Lenth's and Bayes' approaches enabled to determine the effect of every variable even though no degrees of freedom were left to estimate the residual error. The same conclusion about the robustness of the extraction step was reached from the three methodologies, namely, none of the seven factors examined influenced on the method performance significantly, so the sample preparation procedure was considered to be robust.
PB Elsevier
SN 0169-7439
YR 2016
FD 2016-02
LK http://hdl.handle.net/10259/4256
UL http://hdl.handle.net/10259/4256
LA eng
NO Ministerio deCiencia e Innovación (CTQ2011-26022) and MINECO (CTQ2014-53157-R).
DS Repositorio Institucional de la Universidad de Burgos
RD 18-abr-2024